{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:41Z","timestamp":1784557121805,"version":"3.55.0"},"reference-count":62,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>\n                    Androgens act in concert with vitamin D to influence reabsorption of calcium. However, it is unclear whether androgens directly regulate vitamin D homeostasis or control other cellular events that are related to vitamin D metabolism. To examine whether the expression of vitamin D-related genes in mouse kidney is driven by androgens or androgen-dependent effects, the androgen receptor and other sex steroid receptors were monitored in orchidectomized mice treated with 5\u03b1-dihydrotestosterone (DHT). Our results revealed that exposing orchidectomized mice to DHT inhibited the expression of progesterone receptor (Pgr) with or without estrogen receptor \u03b1 expression, the latter was confirmed by ER-positive (MCF7 and T47D) or -negative (PCT) cells analysis. The loss of Pgr in turn decreased the expression of renal 24-hydroxylase\n                    <jats:italic>via<\/jats:italic>\n                    transcriptional regulation because\n                    <jats:italic>Cyp24a1<\/jats:italic>\n                    gene has a progesterone receptor-binding site on promoter. When male kidneys preferentially hydroxylate 25-hydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    using 24-hydroxylase rather than 25-hydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    -1-alpha hydroxylase, DHT suppressed the Pgr-mediated 24-hydroxylase expression, and it is important to note that DHT increased the blood 25-hydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    levels. These findings uncover an important link between androgens and vitamin D homeostasis and suggest that therapeutic modulation of Pgr may be used to treat vitamin D deficiency and related disorders.\n                  <\/jats:p>","DOI":"10.1530\/jme-17-0187","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T20:35:15Z","timestamp":1517344515000},"page":"159-170","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":14,"title":["5\u03b1-dihydrotestosterone reduces renal Cyp24a1 expression via suppression of progesterone receptor"],"prefix":"10.1530","volume":"60","author":[{"given":"Sang R","family":"Lee","sequence":"first","affiliation":[{"name":"1College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Young","family":"Park","sequence":"additional","affiliation":[{"name":"1College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun","family":"Yang","sequence":"additional","affiliation":[{"name":"2Korean Institute of Oriental Medicine, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geun-Shik","family":"Lee","sequence":"additional","affiliation":[{"name":"3College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beum-Soo","family":"An","sequence":"additional","affiliation":[{"name":"4Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University, Miryang, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bae-kuen","family":"Park","sequence":"additional","affiliation":[{"name":"1College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui-Bae","family":"Jeung","sequence":"additional","affiliation":[{"name":"5College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui-Ju","family":"Hong","sequence":"additional","affiliation":[{"name":"1College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"ref561","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1038\/jid.2015.297","article-title":"Androgens attenuate vitamin D production induced by UVB irradiation of the skin of male mice by an enzymatic mechanism","volume":"135","year":"2015","journal-title":"Journal of Investigative Dermatology"},{"key":"ref511","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1016\/0092-8674(89)90051-2","article-title":"Determinants of target gene specificity for steroid\/thyroid hormone receptors","volume":"57","year":"1989","journal-title":"Cell"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.juro.2010.04.068","article-title":"Fracture types and risk factors in men with prostate cancer on androgen deprivation therapy: a matched cohort study of 19,079 men","volume":"184","year":"2010","journal-title":"Journal of Urology"},{"key":"ref571","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.bbrc.2017.03.114","article-title":"The T47D cell line is an ideal experimental model to elucidate the progesterone-specific effects of a luminal A subtype of breast cancer","volume":"486","year":"2017","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"ref221","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1210\/endo-124-3-1576","article-title":"Androgens directly stimulate proliferation of bone cells in vitro","volume":"124","year":"1989","journal-title":"Endocrinology"},{"key":"ref411","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jsbmb.2003.11.008","article-title":"Differential regulation of the human progesterone receptor gene through an estrogen response element half site and Sp1 sites","volume":"88","year":"2004","journal-title":"Journal of Steroid Biochemistry and Molecular Biology"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"17975","DOI":"10.1073\/pnas.1315319110","article-title":"Loss of estrogen-related receptor alpha promotes hepatocarcinogenesis development via metabolic and inflammatory disturbances","volume":"110","year":"2013","journal-title":"PNAS"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1097\/FPC.0b013e32810f2e58","article-title":"Reverse transcriptase-PCR quantification of mRNA levels from cytochrome (CYP)1, CYP2 and CYP3 families in 22 different human tissues","volume":"17","year":"2007","journal-title":"Pharmacogenetics and Genomics"},{"key":"ref591","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1096\/fj.99-0863com","article-title":"Testosterone inhibits estrogen-induced mammary epithelial proliferation and suppresses estrogen receptor expression","volume":"14","year":"2000","journal-title":"FASEB Journal"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"6112","DOI":"10.1073\/pnas.92.13.6112","article-title":"A possible role of vitamin D receptors in regulating vitamin D activation in the kidney","volume":"92","year":"1995","journal-title":"PNAS"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1038\/nrc2196","article-title":"Vitamin D signalling pathways in cancer: potential for anticancer therapeutics","volume":"7","year":"2007","journal-title":"Nature Reviews Cancer"},{"key":"ref251","first-page":"225","article-title":"Metabolic heterogeneity of the nephron","volume":"7","year":"1982","journal-title":"Mineral and Electrolyte Metabolism"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1677\/joe.0.1150295","article-title":"Evidence for an interaction of insulin and sex steroids in the regulation of vitamin D metabolism in the rat","volume":"115","year":"1987","journal-title":"Journal of Endocrinology"},{"key":"ref111","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1097\/01.GME.0000055522.67459.89","article-title":"A physiologic role for testosterone in limiting estrogenic stimulation of the breast","volume":"10","year":"2003","journal-title":"Menopause"},{"key":"ref421","first-page":"235","article-title":"Influence of estrogen on renal vitamin hydroxylases serum alpha OH in chicks of","volume":"25","year":"1978","journal-title":"American Journal Physiology"},{"key":"ref361","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.3390\/nu2091026","article-title":"Experimental evidence for the effects of calcium and vitamin D on bone: a review","volume":"2","year":"2010","journal-title":"Nutrients"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1210\/mend-1-8-535","article-title":"Mouse kidney androgen-regulated protein messenger ribonucleic acid is expressed in the proximal convoluted tubules","volume":"1","year":"1987","journal-title":"Molecular Endocrinology"},{"key":"ref551","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1128\/MCB.8.5.2117","article-title":"mRNA synthesis rates in vivo for androgen-inducible sequences in mouse kidney","volume":"8","year":"1988","journal-title":"Molecular and Cellular Biology"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1677\/joe.0.1750349","article-title":"Expression of the progesterone receptor and progesterone- metabolising enzymes in the female and male human kidney","volume":"175","year":"2002","journal-title":"Journal of Endocrinology"},{"key":"ref281","first-page":"3071","article-title":"MCF-7: the first hormone-responsive breast cancer cell line","volume":"57","year":"1997","journal-title":"Cancer Research"},{"key":"ref331","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1210\/en.2004-1116","article-title":"Altered pharmacokinetics of 1alpha,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3 in the blood and tissues of the 25-hydroxyvitamin D-24-hydroxylase (Cyp24a1) null 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estrogens","author":"ZumoffB","year":"1982","journal-title":"Cancer Research"},{"key":"ref321","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/0092-8674(95)90199-X","article-title":"The nuclear receptor superfamily: the second decade","volume":"83","year":"1995","journal-title":"Cell"},{"key":"ref581","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1210\/endo.138.8.5328","article-title":"1alpha,25-dihydroxyvitamin D3 actions in LNCaP human prostate cancer cells are androgen-dependent","volume":"138","year":"1997","journal-title":"Endocrinology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev.ph.51.030189.000411","article-title":"Androgen-regulated gene expression","volume":"51","year":"1989","journal-title":"Annual Review of Physiology"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1242\/jcs.104.3.695","article-title":"Establishment of renal proximal tubule cell lines by targeted oncogenesis in transgenic mice using the L-pyruvate kinase-SV40 (T) antigen hybrid gene","volume":"104","year":"1993","journal-title":"Journal of Cell Science"},{"key":"ref451","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1016\/S0022-5347(05)65060-4","article-title":"Osteoporosis in men treated with androgen deprivation therapy for prostate cancer","volume":"167","year":"2002","journal-title":"Journal of Urology"},{"key":"ref471","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1042\/bj20011807","article-title":"Hormone-specific regulation of the kidney androgen-regulated gene promoter in cultured mouse renal proximal-tubule cells","volume":"366","year":"2002","journal-title":"Biochemical Journal"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.mce.2005.03.002","article-title":"Calbindin-D28k (CaBP28k) identification and regulation by 1,25-dihydroxyvitamin D3 in human choriocarcinoma cell line 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Diseases"},{"key":"ref601","doi-asserted-by":"crossref","first-page":"15650","DOI":"10.1073\/pnas.1315006110","article-title":"CYP2R1 is a major, but not exclusive, contributor to 25-hydroxyvitamin D production in vivo","volume":"110","year":"2013","journal-title":"PNAS"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1093\/jn\/119.5.741","article-title":"Vitamin D is necessary for reproductive functions of the male rat","volume":"119","year":"1989","journal-title":"Journal of Nutrition"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"4583","DOI":"10.1210\/en.2002-220369","article-title":"Estrogen receptor alpha and activating protein-1 mediate estrogen responsiveness of the progesterone receptor gene in MCF-7 breast cancer cells","volume":"143","year":"2002","journal-title":"Endocrinology"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.chembiol.2013.12.016","article-title":"Vitamin D metabolism, mechanism of action, and clinical applications","volume":"21","year":"2014","journal-title":"Chemical Biology"},{"key":"ref391","doi-asserted-by":"crossref","first-page":"6131","DOI":"10.1158\/0008-5472.CAN-09-0452","article-title":"Androgen receptor inhibits estrogen receptor-alpha activity and is prognostic in breast cancer","volume":"69","year":"2009","journal-title":"Cancer Research"},{"key":"ref521","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1359\/jbmr.2003.18.10.1725","article-title":"Intestinal calcium transporter genes are upregulated by estrogens and the reproductive cycle through vitamin D receptor-independent mechanisms","volume":"18","year":"2003","journal-title":"Journal of Bone and Mineral Research"},{"key":"ref131","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1210\/jc.2010-1628","article-title":"ForestaCStrapazzonGDe mineral density and testicular failure : evidence for a role of vitamin hydroxylase in human testis of and https org","volume":"25","author":"ToniLPerilliLDi","year":"2011","journal-title":"Bone Journal Clinical Endocrinology Metabolism"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1002\/mrd.22033","article-title":"Calcium transport genes are differently regulated in maternal and fetal placenta in the knockout mice of calbindin-D(9k) and -D(28k)","volume":"79","year":"2012","journal-title":"Molecular Reproduction and Development"},{"key":"ref481","doi-asserted-by":"crossref","first-page":"2701","DOI":"10.1073\/pnas.73.8.2701","article-title":"Control of renal vitamin D hydroxylases in birds by sex hormones","volume":"73","year":"1976","journal-title":"PNAS"},{"key":"ref211","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1152\/physrev.1998.78.4.1193","article-title":"Current understanding of the molecular actions of vitamin D","volume":"78","year":"1998","journal-title":"Physiological Reviews"},{"key":"ref271","first-page":"2597","article-title":"Genistein and 17beta-estradiol, but not equol, regulate vitamin D synthesis in human colon and breast cancer cells","volume":"26","year":"2006","journal-title":"Anticancer Research"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1210\/mend-4-8-1240","article-title":"Cell-specific expression of kidney androgen-regulated protein messenger RNA is under multihormonal control","volume":"4","year":"1990","journal-title":"Molecular Endocrinology"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/pros.20189","article-title":"5alpha-dihydrotestosterone inhibits 1alpha,25-dihydroxyvitamin D3-induced expression of CYP24 in human prostate cancer cells","volume":"63","year":"2005","journal-title":"Prostate"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"6410","DOI":"10.1210\/jc.2005-0183","article-title":"Bone loss after initiation of androgen deprivation therapy in patients with prostate cancer","volume":"90","year":"2005","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1210\/endo-122-3-935","article-title":"Regulation of progesterone receptor messenger ribonucleic acid and protein levels in MCF-7 cells by estradiol: analysis of estrogen\u2019s effect on progesterone receptor synthesis and degradation","volume":"122","year":"1988","journal-title":"Endocrinology"},{"key":"ref441","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1210\/mend-2-3-263","article-title":"Ligand-modulated regulation of progesterone receptor messenger ribonucleic acid and protein in human breast cancer cell lines","volume":"2","year":"1988","journal-title":"Molecular Endocrinology"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/ejcn.2011.140","article-title":"Impact of gender on vitamin D deficiency in morbidly obese patients: a cross-sectional study","volume":"66","year":"2012","journal-title":"European Journal of Clinical Nutrition"},{"key":"ref501","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/0092-8674(79)90170-3","article-title":"An abundant androgen-regulated mRNA in the mouse kidney","volume":"17","year":"1979","journal-title":"Cell"},{"key":"ref81","first-page":"71","article-title":"Regulation of gene expression by androgens in murine kidney","volume":"42","year":"1986","journal-title":"Recent Progress in Hormone Research"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1210\/me.2011-1355","article-title":"Research resource: genome-wide profiling of progesterone receptor binding in the mouse uterus","volume":"26","year":"2012","journal-title":"Molecular Endocrinology"},{"key":"ref121","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1016\/S0140-6736(10)61916-2","article-title":"Testiculopathy and vitamin D insufficiency","volume":"376","year":"2010","journal-title":"Lancet"},{"key":"ref151","doi-asserted-by":"crossref","first-page":"23330","DOI":"10.3390\/ijms141223330","article-title":"Biological significance of calbindin-D9k within duodenal epithelium","volume":"14","year":"2013","journal-title":"International Journal of Molecular Sciences"},{"key":"ref431","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1210\/endo-125-1-392","article-title":"Down-regulation of estrogen receptors by androgens in the ZR-75-1 human breast cancer cell line","volume":"125","year":"1989","journal-title":"Endocrinology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1126\/science.7010603","article-title":"Testosterone: a major determinant of extragenital sexual dimorphism","volume":"211","year":"1981","journal-title":"Science"},{"key":"ref161","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1210\/me.2010-0483","article-title":"Cytoplasmic accumulation of 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