{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:23Z","timestamp":1784557103545,"version":"3.55.0"},"reference-count":36,"publisher":"Bioscientifica","issue":"2","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>\n                    In women, establishment of pregnancy is dependent upon \u2018fine-tuning\u2019 of the endometrial microenvironment, which is mediated by terminal differentiation (decidualisation) of endometrial stromal fibroblasts (ESFs). We have demonstrated that intracrine steroid metabolism plays a key role in regulating decidualisation and is essential for time-dependent expression of key factors required for endometrial receptivity. The primary aim of the current study was to determine whether sulphated steroids can act as precursors to bioactive sex steroids during decidualisation. We used primary human ESF and a robust\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of decidualisation to assess the expression of genes associated with sulphation, desulphation and transport of sulphated steroids in human ESF as well as the impact of the steroid sulphatase (STS) inhibitor STX64 (Irosustat). We found evidence for an increase in both expression and activity of STS in response to a decidualisation stimulus with abrogation of oestrone biosynthesis and decreased secretion of the decidualisation marker IGFBP1 in the presence of STX64. These results provide novel insight into the contribution of STS to the intracrine regulation of decidualisation.\n                  <\/jats:p>","DOI":"10.1530\/jme-18-0037","type":"journal-article","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T11:10:14Z","timestamp":1525259414000},"page":"M57-M65","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":8,"title":["SULFATION PATHWAYS: A role for steroid sulphatase in intracrine regulation of endometrial decidualisation"],"prefix":"10.1530","volume":"61","author":[{"given":"Douglas A","family":"Gibson","sequence":"first","affiliation":[{"name":"1MRC Centre for Inflammation Research, The University of Edinburgh, QMRI, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A","family":"Foster","sequence":"additional","affiliation":[{"name":"2Institute of Metabolism & Systems Research, University of Birmingham, Birmingham, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Simitsidellis","sequence":"additional","affiliation":[{"name":"1MRC Centre for Inflammation Research, The University of Edinburgh, QMRI, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary O D","family":"Critchley","sequence":"additional","affiliation":[{"name":"3MRC Centre for Reproductive Health, The University of Edinburgh, QMRI, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olympia","family":"Kelepouri","sequence":"additional","affiliation":[{"name":"1MRC Centre for Inflammation Research, The University of Edinburgh, QMRI, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances","family":"Collins","sequence":"additional","affiliation":[{"name":"1MRC Centre for Inflammation Research, The University of Edinburgh, QMRI, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippa T K","family":"Saunders","sequence":"additional","affiliation":[{"name":"1MRC Centre for Inflammation Research, The University of Edinburgh, QMRI, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"ref31","doi-asserted-by":"crossref","first-page":"19622","DOI":"10.1074\/jbc.M111.297663","article-title":"Estrogen-induced expression of Fos-related antigen 1 (FRA-1) regulates uterine stromal differentiation and remodeling","volume":"287","year":"2012","journal-title":"Journal of Biological Chemistry"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1210\/er.2014-1045","article-title":"Cyclic decidualization of the human endometrium in reproductive health and failure","volume":"35","year":"2014","journal-title":"Endocrine Reviews"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.mce.2011.06.012","article-title":"Steroid sulfatase: a pivotal player in estrogen synthesis and metabolism","volume":"340","year":"2011","journal-title":"Molecular and Cellular Endocrinology"},{"key":"ref01","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1210\/jc.2010-0154","article-title":"role for the orphan nuclear receptor estrogen - 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