{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:22:22Z","timestamp":1790839342320,"version":"4.1.0"},"reference-count":110,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,10]]},"abstract":"\n Multiple signaling molecules and transcription factors are required for pituitary development. Activator-type bHLH genes\n Mash1<\/jats:italic>\n ,\n Math<\/jats:italic>\n ,\n NeuroD (Neurod)<\/jats:italic>\n and Neurogenin\n (Neurog)<\/jats:italic>\n are well known as key molecules in neural development. Although analyses of targeted mouse mutants have demonstrated involvement of these bHLH genes in pituitary development, studies with single-mutant mice could not elucidate their exact functions, because they cooperatively function and compensate each other. The aim of this study was to elucidate the roles of\n Mash1<\/jats:italic>\n ,\n Math3<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n in pituitary development.\n Mash1<\/jats:italic>\n ;\n Math3<\/jats:italic>\n ;\n NeuroD<\/jats:italic>\n triple-mutant mice were analyzed by immunohistochemistry and quantitative real-time RT-PCR. Misexpression studies with retroviruses in pituisphere cultures were also performed. The triple-mutant adenohypophysis was morphologically normal, though the lumen of the neurohypophysis remained unclosed. However, in triple-mutant pituitaries, somatotropes, gonadotropes and corticotropes were severely decreased, whereas lactotropes were increased. Misexpression of\n Mash1<\/jats:italic>\n alone with retrovirus could not induce generation of hormonal cells, though\n Mash1<\/jats:italic>\n was involved in differentiation of pituitary progenitor cells. These data suggest that\n Mash1<\/jats:italic>\n ,\n Math3<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n cooperatively control the timing of pituitary progenitor cell differentiation and that they are also required for subtype specification of pituitary hormonal cells.\n Mash1<\/jats:italic>\n is necessary for corticotroph and gonadotroph differentiation, and compensated by\n Math3<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n .\n Math3<\/jats:italic>\n is necessary for somatotroph differentiation, and compensated by\n Mash1<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n .\n Neurog2<\/jats:italic>\n may compensate\n Mash1<\/jats:italic>\n ,\n Math3<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n during pituitary development. Furthermore,\n Mash1<\/jats:italic>\n ,\n Math3<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n are required for neurohypophysis development. Thus,\n Mash1<\/jats:italic>\n ,\n Math3<\/jats:italic>\n and\n NeuroD<\/jats:italic>\n are required for pituitary development, and compensate each other.\n <\/jats:p>","DOI":"10.1530\/jme-18-0090","type":"journal-article","created":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T11:25:55Z","timestamp":1529925955000},"page":"127-138","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":27,"title":["The proneural bHLH genes Mash1, Math3 and NeuroD are required for pituitary development"],"prefix":"10.1530","volume":"61","author":[{"given":"Mitsushige","family":"Ando","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Goto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Hojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Kita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Kitagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Ohtsuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoichiro","family":"Kageyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"ref661","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1242\/dev.128.8.1313","article-title":"Roles 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