{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:25Z","timestamp":1784557105330,"version":"3.55.0"},"reference-count":35,"publisher":"Bioscientifica","issue":"4","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>\n                    Human corneal fibroblasts (HCFs) are implicated in corneal neovascularization (CRNV). The mechanisms underlying the inflammatory response in HCFs and the development of CRNV were explored in this study. Alkali burns were applied to the corneas of rats to establish a CRNV model. The expression of long noncoding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) and mRNA and protein levels of nuclear factor kappa B (NF-\u03baB)- activating protein (NKAP) were examined by quantitative real-time (qRT-PCR) and Western blot methods, respectively. Lipopolysaccharide (LPS) is used to stimulate HCFs for inflammatory response. The level of inflammation factors in HCF supernatant was detected using an enzyme-linked immunosorbent assay (ELISA). Binding and interactions between NEAT1 and miRNA 1246 (miR-1246) were determined by RNA immunoprecipitation (RIP) and RNA pull-down assays in HCFs. Compared with the control group (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096), NEAT1 was upregulated in the corneas of the CRNV rat model (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096). The expression of NEAT1 in HCFs was upregulated by LPS. Downregulation of NEAT1 suppressed the secretion of tumor necrosis factor-\u03b1 (TNF-\u03b1) and interleukin-6 (IL-6). NEAT1 could bind and interact with miR-1246. LPS regulated the expression of NKAP and NF-\u03baB signaling via the NEAT1\/miR-1246 pathway. Downregulation of NEAT1\n                    <jats:italic>in vivo<\/jats:italic>\n                    inhibited CRNV progression in the CRNV rat model. The lncRNA NEAT1 induced secretion of inflammatory factors, mediated by NF-\u03baB, by targeting miR-1246, thereby promoting CRNV progression.\n                  <\/jats:p>","DOI":"10.1530\/jme-18-0098","type":"journal-article","created":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T06:25:41Z","timestamp":1539239141000},"page":"231-239","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":43,"title":["LncRNA NEAT1 promotes inflammatory response and induces corneal neovascularization"],"prefix":"10.1530","volume":"61","author":[{"given":"Yan-hui","family":"Bai","sequence":"first","affiliation":[{"name":"1Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Lv","sequence":"additional","affiliation":[{"name":"1Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-qun","family":"Wang","sequence":"additional","affiliation":[{"name":"1Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-li","family":"Sun","sequence":"additional","affiliation":[{"name":"1Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"2Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"ref231","doi-asserted-by":"crossref","first-page":"5871","DOI":"10.1167\/iovs.14-14433","article-title":"Inhibition of lymphangiogenesis and hemangiogenesis in corneal inflammation by subconjunctival Prox1 siRNA injection in rats","volume":"56","year":"2015","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1600-065X.2012.01099.x","article-title":"NF-kappaB and the link between inflammation and cancer","volume":"246","year":"2012","journal-title":"Immunological Reviews"},{"key":"ref301","first-page":"901315","article-title":"Toona sinensis inhibits LPS-induced inflammation and migration in vascular smooth muscle cells via suppression of reactive oxygen species and NF-kappaB signaling pathway","volume":"2014","year":"2014","journal-title":"Oxidative Medicine and Cellular 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