{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:14:39Z","timestamp":1783527279726,"version":"3.55.0"},"reference-count":59,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:p>Fertility is dependent on follicle-stimulating hormone (FSH), a product of gonadotrope cells of the anterior pituitary gland. Hypothalamic gonadotropin-releasing hormone (GnRH) and intra-pituitary activins are regarded as the primary drivers of FSH synthesis and secretion. Both stimulate expression of the FSH beta subunit gene (Fshb), although the underlying mechanisms of GnRH action are poorly described relative to those of the activins. There is currently no consensus on how GnRH regulates Fshb transcription, as results vary across species and between in vivo and in vitro approaches. One of the more fully developed models suggests that the murine Fshb promoter is tonically repressed by histone deacetylases (HDACs) and that GnRH relieves this repression, at least in immortalized murine gonadotrope-like cells (L\u03b2T2 and \u03b1T3-1). In contrast, we observed that the class I\/II HDAC inhibitor trichostatin A (TSA) robustly inhibited basal, activin A-, and GnRH-induced Fshb mRNA expression in L\u03b2T2 cells and in primary murine pituitary cultures. Similar results were obtained with the class I specific HDAC inhibitor, entinostat, whereas two class II-specific inhibitors, MC1568 and TMP269, had no effects on Fshb expression. Collectively, these data suggest that class I HDACs are positive, not negative, regulators of Fshb expression in vitro and that, contrary to earlier reports, GnRH may not stimulate Fshb by inhibiting HDAC-mediated repression of the gene.<\/jats:p>","DOI":"10.1530\/jme-18-0145","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T12:04:38Z","timestamp":1543320278000},"page":"67-78","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":9,"title":["HDAC inhibitors impair\n                    <i>Fshb<\/i>\n                    subunit expression in murine gonadotrope cells"],"prefix":"10.1530","volume":"62","author":[{"given":"Gauthier","family":"Schang","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics 1","department":["Centre for Research in Reproduction and Development, McGill University, Montreal, Quebec, 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