{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:20Z","timestamp":1784557220957,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"4","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>Physiological or supraphysiological levels of testosterone appear to be associated with the development of risk factors for cardiovascular diseases such as hypertension, as this hormone modulates the release of endothelial factors. However, its actions are still controversial, especially in the coronary circulation of hypertensive animals. This study was designed to assess the effects of testosterone treatment (T) on endothelium-dependent coronary vascular reactivity in orchiectomized SHR. The animals were divided into SHAM, orchiectomized (ORX), ORX+T and ORX+T+aromatase inhibitor (AI). All treatments lasted 15 days. Blood pressure (BP) was measured. Dose\u2013response curves to bradykinin (BK) were constructed using the Langendorff technique, followed by inhibition of endothelium mediators (NO, prostanoids, EETs) and potassium channels. The intensity of eNOS, COX-1, COX-2, Akt, and gp91phox protein expression was quantified by Western blotting. BP was elevated in SHAM, ORX+T, and ORX+T+AI groups. However, we did not observe differences in the ORX group. Baseline coronary perfusion pressure (CPP) remained unaffected. Orchiectomy did not change the BK-induced relaxation compared to the SHAM group, whereas testosterone treatment increased it. This response was diminished in the absence of NO, prostanoids, and EETs in the SHAM and ORX groups, while in ORX+T group the relaxation was diminished only in the absence of NO and EETs. There was no difference in eNOS, COX-1, COX-2, and gp91phox protein expression, though Akt expression was increased in ORX and ORX+T groups. These results show that testosterone treatment can modulate endothelial function, especially in the coronary circulation under hypertension conditions, via NO and EETs pathways.<\/jats:p>","DOI":"10.1530\/jme-20-0153","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T11:16:28Z","timestamp":1599218188000},"page":"125-134","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":5,"title":["Testosterone increases bradykinin-induced relaxation in the coronary bed of hypertensive rats"],"prefix":"10.1530","volume":"65","author":[{"given":"Juan Carlos","family":"Arapa-Diaz","sequence":"first","affiliation":[{"name":"1Department of Physiological Sciences, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wender do Nascimento","family":"Rouver","sequence":"additional","affiliation":[{"name":"1Department of Physiological Sciences, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssyca Aparecida Soares","family":"Giesen","sequence":"additional","affiliation":[{"name":"1Department of Physiological Sciences, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Daruge","family":"Grando","sequence":"additional","affiliation":[{"name":"2Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences from Ribeir\u00e3o Preto, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lusiane Maria","family":"Bendhack","sequence":"additional","affiliation":[{"name":"2Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences from Ribeir\u00e3o Preto, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger Lyrio dos","family":"Santos","sequence":"additional","affiliation":[{"name":"1Department of Physiological Sciences, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/65\/4\/JME-20-0153.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jme.bioscientifica.com\/downloadpdf\/journals\/jme\/65\/4\/JME-20-0153.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:53:58Z","timestamp":1784555638000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/jme\/article\/65\/4\/125-134\/14447"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1530\/jme-20-0153","relation":{},"ISSN":["0952-5041","1479-6813"],"issn-type":[{"value":"0952-5041","type":"print"},{"value":"1479-6813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11]]}}}