{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:54:42Z","timestamp":1790434482279,"version":"4.1.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    Asprosin is a novel fasting-induced protein encoded by fibrillin-1 (\n                    <jats:italic>FBN1<\/jats:italic>\n                    ) gene, produced when FBN1 is cleaved by the enzyme furin, and is associated with insulin resistance and polycystic ovarian syndrome in humans. To characterize mRNA abundance of\n                    <jats:italic>FBN1<\/jats:italic>\n                    , FURIN, and the presumed asprosin receptor, olfactory receptor family 4 subfamily M member 1 (\n                    <jats:italic>OR4M1<\/jats:italic>\n                    ) in granulosa (GC) and theca cells (TC), and identify hormones regulating\n                    <jats:italic>FBN1<\/jats:italic>\n                    mRNA expression, GC and TC from small (1\u20135 mm; SM) and large (&gt;8 mm; LG) follicles were collected from ovaries of heifers obtained at an abattoir and used for real-time PCR gene expression analysis or\n                    <jats:italic>in vitro<\/jats:italic>\n                    evaluation of hormone regulation and asprosin effects. SMTC had 151-fold greater (\n                    <jats:italic>P &lt;<\/jats:italic>\n                    0.05)\n                    <jats:italic>FBN1<\/jats:italic>\n                    mRNA abundance than SMGC, and LGTC had 50-fold greater\n                    <jats:italic>FBN1<\/jats:italic>\n                    mRNA than LGGC. In contrast,\n                    <jats:italic>OR4M1<\/jats:italic>\n                    mRNA was 81-fold greater in SMGC than LGGC and did not differ from SMTC, but LGTC had 9-fold greater\n                    <jats:italic>OR4M1<\/jats:italic>\n                    mRNA than LGGC. FURIN mRNA was 2.6-fold greater in SMTC than SMGC, but did not differ among follicular sizes. In cultured TC, leptin, insulin, LH, IGF1 and steroids did not affect\n                    <jats:italic>FBN1<\/jats:italic>\n                    mRNA, but TGFB1 increased (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05)\n                    <jats:italic>FBN1<\/jats:italic>\n                    mRNA by 2.2-fold; EGF and FGFs increased\n                    <jats:italic>FBN1<\/jats:italic>\n                    mRNA by 1.3- to 1.5-fold. Asprosin enhanced LH-induced TC androstenedione production, reduced IGF1-induced TC proliferation, and had no effect on progesterone production. Developmental regulation of\n                    <jats:italic>FBN1<\/jats:italic>\n                    , FURIN and\n                    <jats:italic>OR4M1<\/jats:italic>\n                    along with direct effects of asprosin on TC suggests that asprosin may be a novel regulator of ovarian follicular function.\n                  <\/jats:p>","DOI":"10.1530\/jme-20-0218","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T18:16:14Z","timestamp":1603217774000},"page":"35-44","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":36,"title":["Discovery of a possible role of asprosin in ovarian follicular function"],"prefix":"10.1530","volume":"66","author":[{"given":"Excel Rio S","family":"Maylem","sequence":"first","affiliation":[{"name":"1Department of Animal and Food Sciences, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon J","family":"Spicer","sequence":"additional","affiliation":[{"name":"1Department of Animal and Food Sciences, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora","family":"Batalha","sequence":"additional","affiliation":[{"name":"2Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis F","family":"Schutz","sequence":"additional","affiliation":[{"name":"2Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno, Nevada, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/66\/1\/JME-20-0218.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jme.bioscientifica.com\/downloadpdf\/journals\/jme\/66\/1\/JME-20-0218.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:54:00Z","timestamp":1784555640000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/jme\/article\/66\/1\/35-44\/14442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/jme-20-0218","relation":{},"ISSN":["0952-5041","1479-6813"],"issn-type":[{"value":"0952-5041","type":"print"},{"value":"1479-6813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]}}}