{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:32Z","timestamp":1784557232993,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1,1]]},"abstract":"<jats:p>\n                    Increased inflammatory response is one of the major characteristics of osteonecrosis of the femoral head (ONFH). We aimed to investigate the function of bone morphogenetic protein 2 (BMP-2)\/interleukin (IL)-34 axis in the inflammatory responses of ONFH. The systemic and local expression of BMPs in ONFH patients was detected by qRT-PCR and ELISA.\n                    <jats:italic>In vitro<\/jats:italic>\n                    osteoclast differentiation and ONFH mouse models, induced by 20 mg\/kg methylprednisolone through i.m. injection, were established using WT and BMP-2\n                    <jats:sup>\n                      <jats:bold>\u2212<\/jats:bold>\n                      \/\n                      <jats:bold>\u2212<\/jats:bold>\n                    <\/jats:sup>\n                    mice to explore the regulatory role of BMP-2 in pro-inflammatory responses and bone defects of ONFH. IL-34 expression and function were examined\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    through qRT-PCR, tartrate-resistant acid phosphatase (TRAP) staining, and gene knockout. The systemic and local expression of BMPs was elevated in ONFH patients. BMP-2 reduced the production of pro-inflammatory cytokines and inhibited the differentiation of osteoclasts. Mechanistically, BMP-2 inhibited osteoclasts formation through suppressing IL-34 expression and then promoted bone repair and alleviated ONFH. In conclusion, our study reveals that BMP-2 inhibits inflammatory responses and osteoclast formation through downregulating IL-34.\n                  <\/jats:p>","DOI":"10.1530\/jme-21-0163","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T14:18:50Z","timestamp":1632838730000},"page":"1-9","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":7,"title":["Bone morphogenetic protein 2 controls steroid-induced osteonecrosis of the femoral head via directly inhibiting interleukin-34 expression"],"prefix":"10.1530","volume":"68","author":[{"given":"Meng","family":"Wang","sequence":"first","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Sung","family":"Min","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Shan","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Lin","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyang","family":"Xia","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuli","family":"Yin","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaolai","family":"Jiang","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Yu","sequence":"additional","affiliation":[{"name":"1Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/68\/1\/JME-21-0163.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jme.bioscientifica.com\/downloadpdf\/journals\/jme\/68\/1\/JME-21-0163.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:54:07Z","timestamp":1784555647000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/jme\/article\/68\/1\/1-9\/14543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/jme-21-0163","relation":{},"ISSN":["0952-5041","1479-6813"],"issn-type":[{"value":"0952-5041","type":"print"},{"value":"1479-6813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,1]]}}}