{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:30Z","timestamp":1784557230691,"version":"3.55.0"},"reference-count":51,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5,31]]},"abstract":"<jats:p>\n                    Arylalkylamine\n                    <jats:italic>N<\/jats:italic>\n                    -acetyltransferase (AANAT), a rate-limiting enzyme in melatonin synthesis, is present in extra-pineal tissues such as the hippocampus. The hippocampal AANAT activity in amyloid \u03b2 (A\u03b2) neurotoxicity has not been exactly defined. Adult male rats received bilateral intra-CA1 A\u03b2 administration. The hippocampus tissue sampling was performed 2, 12, and 24 h after A\u03b2 injection in the morning and night. The inflammation was monitored using tumor necrosis factor-alpha (TNF-\u03b1) immunohistochemistry. The AANAT enzyme activity and melatonin levels were measured using western blotting and high-performance liquid chromatography. The sampling in the morning vs night showed no significant differences in the AANAT activity. The A\u03b2 increased the area of TNF-\u03b1 positive staining 24 h after injection, which indicated the induction of an inflammatory context. It was accompanied by a significant reduction in AANAT activity and hippocampal melatonin. A reverse correlation was also detected between TNF-\u03b1 and AANAT activity in the 24-h group. The TNF-\u03b1 positive area was significantly increased in the 24-h group as compared to the 12-h group. Data showed that inflammatory processes began 12 h after the A\u03b2 injection and augmented 24 h later. In the second experiment, the impact of A\u03b2 injection on hippocampus AANAT activity was examined in the pinealectomized (PIN\u00d7) animals. The PIN\u00d7 per se did not affect the hippocampal AANAT and melatonin levels. However, there was a significant decrease in hippocampal melatonin in the PIN\u00d7+A\u03b2 group. The findings suggest the accompanying hippocampal inflammatory context and AANAT enzyme activity reduction in early stages after A\u03b2 administration. Understanding the underlying mechanism of the decreased AANAT activity may suggest new treatment strategies.\n                  <\/jats:p>","DOI":"10.1530\/jme-22-0161","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T11:25:53Z","timestamp":1685532353000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of hippocampal arylalkylamine N-acetyltransferase activity in amyloid-\u03b2 neurotoxicity"],"prefix":"10.1530","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5199-5958","authenticated-orcid":true,"given":"Shima","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8950-893X","authenticated-orcid":true,"given":"Maryam","family":"Zahmatkesh","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"},{"name":"Research Center for Cognitive and Behavioral Sciences, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6993-6956","authenticated-orcid":true,"given":"Yazdan","family":"Asgari","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2526-2870","authenticated-orcid":true,"given":"Samaneh","family":"Aminyavari","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0724-6479","authenticated-orcid":true,"given":"Gholamreza","family":"Hassanzadeh","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Biocell","first-page":"265","article-title":"Standardization of fixation, processing and staining methods for the central nervous system of vertebrates","volume":"20","author":"Aldana Marcos","year":"1996","unstructured":"Aldana Marcos HJFerrari CCBenitez I & Affanni JM1996Standardization of fixation, processing and staining methods for the central nervous system of vertebrates. 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