{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:03Z","timestamp":1784557143072,"version":"3.55.0"},"reference-count":29,"publisher":"Bioscientifica","issue":"3","license":[{"start":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:00:00Z","timestamp":1704326400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,1,4]]},"abstract":"<jats:p>\n                    Extra-adrenal\n                    <jats:italic>de novo<\/jats:italic>\n                    aldosterone (Aldo) production has been described inconsistently. Systematic data based upon state-of-the-art technology including validated controls are sparse. We hypothesized that aldosterone synthase (\n                    <jats:italic>CYP11B2<\/jats:italic>\n                    ) expression and\n                    <jats:italic>de novo<\/jats:italic>\n                    Aldo production are absent in nonadrenal human cell lines, either immortalized cell lines or commercially available primary cell lines, including peripheral blood mononuclear cells (PBMCs) of individuals without and with primary hyperaldosteronism (PA). CYP11B2-transfected COS-7 and endogenous CYP11B2 expressing adrenal H295R cells served as positive controls. Various well-characterized, purchased, immortalized (BeWo, HEK293, HTR-8\/SVneo, JEG-3) and primary (HAEC, HLEC, HRGEC, HRMC, HUAEC, HUVEC, PBMC) cell lines as well as self-isolated PBMCs from PA patients (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) were incubated with the steroid hormone substrates progesterone, deoxycorticosterone, corticosterone or 18-OH-corticosterone with and without Ang II for 24 h to assess CYP11B2 enzymatic activity.\n                    <jats:italic>CYP11B2<\/jats:italic>\n                    expression was analyzed by real-time PCR and liquid chromatography\u2013mass spectrometry was used to quantify Aldo production. Pronounced\n                    <jats:italic>CYP11B2<\/jats:italic>\n                    mRNA expression and Aldo production were observed in both positive controls, which followed an incremental time course. Neither substrates alone nor coincubation with Ang II significantly stimulated\n                    <jats:italic>CYP11B2<\/jats:italic>\n                    expression or Aldo production in various immortalized and primary cell lines and PBMCs of PA patients. These results strongly support the absence of relevant\n                    <jats:italic>de novo<\/jats:italic>\n                    extra-adrenal Aldo production in nonadrenal cells, including blood mononuclear cells, irrespective of the absence or presence of autonomous adrenal Aldo production.\n                  <\/jats:p>","DOI":"10.1530\/jme-23-0100","type":"journal-article","created":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T14:14:23Z","timestamp":1704377663000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":2,"title":["No extra-adrenal aldosterone production in various human cell lines"],"prefix":"10.1530","volume":"72","author":[{"given":"Isabelle","family":"Durrer","sequence":"first","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahel","family":"Klossner","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"},{"name":"Department of Internal Medicine, Sonnenhof, Lindenhofgruppe, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Gr\u00f6ssl","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa","family":"V\u00f6gel","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Therina","family":"Du Toit","sequence":"additional","affiliation":[{"name":"Department for BioMedical Research University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Vogt","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Jamin","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"},{"name":"Department for BioMedical Research University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus G","family":"Mohaupt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Sonnenhof, Lindenhofgruppe, Berne, Switzerland"},{"name":"Department for BioMedical Research University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7386-5935","authenticated-orcid":true,"given":"Carine","family":"Gennari-Moser","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension University of Bern, Berne, Switzerland"},{"name":"Department for BioMedical Research University of Bern, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"PLoS One","first-page":"e0269920","article-title":"Increased glucocorticoid metabolism in diabetic kidney disease","volume":"17","author":"Ackermann","year":"2022","unstructured":"Ackermann DVogt BBochud MBurnier MMartin PYPaccaud FEhret GGuessous IPonte BPruijm M, et al.2022Increased glucocorticoid metabolism in diabetic kidney disease. 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