{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:55Z","timestamp":1784557195486,"version":"3.55.0"},"reference-count":48,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p>\n                    GnRH is the main regulator of the hypothalamic\u2013pituitary\u2013gonadal (H\u2013P\u2013G) axis. GnRH stimulates the pituitary gonadotroph to synthesize and secrete gonadotrophins (LH and FSH), and this effect of GnRH is dependent on the availability of glucose and other nutrients. Little is known about whether GnRH regulates glucose metabolism in the gonadotroph. This study examined the regulation of glucose transporters (\n                    <jats:italic>Gluts<\/jats:italic>\n                    ) by GnRH in the L\u03b2T2 gonadotroph cell line. Using real-time PCR analysis, the expression of\n                    <jats:italic>Glut1<\/jats:italic>\n                    , -2, -4, and -8 was detected, but\n                    <jats:italic>Glut1<\/jats:italic>\n                    mRNA expression level was more abundant than the mRNA expression levels of\n                    <jats:italic>Glut2<\/jats:italic>\n                    , -4, and -8. After the treatment of L\u03b2T2 cells with GnRH,\n                    <jats:italic>Glut1<\/jats:italic>\n                    mRNA expression was markedly induced, but there was no GnRH-induction of\n                    <jats:italic>Glut2<\/jats:italic>\n                    , -4, or -8 mRNA expression in L\u03b2T2 cells. The effect of GnRH on\n                    <jats:italic>Glut1<\/jats:italic>\n                    mRNA expression is partly mediated by ERK activation. GnRH increased GLUT1 protein and stimulated GLUT1 translocation to the cell surface of L\u03b2T2 cells. Glucose uptake assays were performed in L\u03b2T2 cells and showed that GnRH stimulates glucose uptake in the gonadotroph. Finally, exogenous treatment of mice with GnRH increased the expression of\n                    <jats:italic>Glut1<\/jats:italic>\n                    but not the expression of\n                    <jats:italic>Glut2<\/jats:italic>\n                    , -4, or -8 in the pituitary. Therefore, regulation of glucose metabolism by GnRH via changes in\n                    <jats:italic>Gluts<\/jats:italic>\n                    expression and subcellular location in the pituitary gonadotroph reveals a novel response of the gonadotroph to GnRH.\n                  <\/jats:p>","DOI":"10.1530\/joe-11-0359","type":"journal-article","created":{"date-parts":[[2011,11,23]],"date-time":"2011-11-23T00:07:24Z","timestamp":1322006844000},"page":"139-147","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":12,"title":["GnRH increases glucose transporter-1 expression and stimulates glucose uptake in the gonadotroph"],"prefix":"10.1530","volume":"212","author":[{"given":"Valerie M","family":"Harris","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachin V","family":"Bendre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francina","family":"Gonzalez De Los Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alemu","family":"Fite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"El-Yaman El-Dandachli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyazat","family":"Kurenbekova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul B","family":"Abou-Samra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen","family":"Buggs-Saxton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"1_41528648","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1750-1326-6-23","volume":"6","year":"2011","journal-title":"MOLECULAR NEURODEGENERATION"},{"issue":"5","key":"2_15998914","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.2527\/1995.7351416x","volume":"73","author":"Barb","year":"1995","journal-title":"Journal of Animal Science","ISSN":"https:\/\/id.crossref.org\/issn\/0021-8812","issn-type":"print"},{"issue":"7","key":"3_34602541","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1210\/me.2009-0030","volume":"23","year":"2009","journal-title":"Molecular Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0888-8809","issn-type":"print"},{"issue":"3","key":"4_37267178","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.yfrne.2010.04.002","volume":"31","author":"Bliss","year":"2010","journal-title":"Frontiers in neuroendocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0091-3022","issn-type":"print"},{"issue":"3","key":"5_9506132","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1677\/joe.0.1290321","volume":"129","author":"Cagampang","year":"1991","journal-title":"Journal of Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0022-0795","issn-type":"print"},{"issue":"1","key":"6_9475672","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1210\/jcem-73-1-35","volume":"73","author":"CAMERON","year":"1991","journal-title":"Journal of Clinical Endocrinology &amp; 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