{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T15:29:53Z","timestamp":1789918193071,"version":"4.0.1"},"reference-count":31,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>\n                    Two forms of prolactin (Prl), prolactin 177 (Prl\n                    <jats:sub>177<\/jats:sub>\n                    ) and prolactin 188 (Prl\n                    <jats:sub>188<\/jats:sub>\n                    ), are produced in the\n                    <jats:italic>rostral pars distalis<\/jats:italic>\n                    (RPD) of the pituitary gland of euryhaline Mozambique tilapia,\n                    <jats:italic>Oreochromis mossambicus<\/jats:italic>\n                    . Consistent with their roles in fresh water (FW) osmoregulation, release of both Prls is rapidly stimulated by hyposmotic stimuli, both\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . We examined the concurrent dynamics of Prl\n                    <jats:sub>177<\/jats:sub>\n                    and Prl\n                    <jats:sub>188<\/jats:sub>\n                    hormone release and mRNA expression from Prl cells in response to changes in environmental salinity\n                    <jats:italic>in vivo<\/jats:italic>\n                    and to changes in extracellular osmolality\n                    <jats:italic>in vitro<\/jats:italic>\n                    . In addition, mRNA levels of Prl receptors 1 and 2 (\n                    <jats:italic>prlr1<\/jats:italic>\n                    and\n                    <jats:italic>prlr2<\/jats:italic>\n                    ) and osmotic stress transcription factor 1 (\n                    <jats:italic>ostf1<\/jats:italic>\n                    ) were measured. Following transfer from seawater (SW) to FW, plasma osmolality decreased, while plasma levels of Prl\n                    <jats:sub>177<\/jats:sub>\n                    and Prl\n                    <jats:sub>188<\/jats:sub>\n                    and RPD mRNA levels of\n                    <jats:italic>prl<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>177<\/jats:italic>\n                    <\/jats:sub>\n                    and\n                    <jats:italic>prl<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>188<\/jats:italic>\n                    <\/jats:sub>\n                    increased. The opposite pattern was observed when fish were transferred from FW to SW. Moreover, hyposmotically induced release of Prl\n                    <jats:sub>188<\/jats:sub>\n                    was greater in Prl cells isolated from FW-acclimated fish after 6\u200ah of incubation, while the hyposmotically induced increase in\n                    <jats:italic>prl<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>188<\/jats:italic>\n                    <\/jats:sub>\n                    mRNA levels was only observed in SW-acclimated fish. In addition,\n                    <jats:italic>prlr2<\/jats:italic>\n                    and\n                    <jats:italic>ostf1<\/jats:italic>\n                    mRNA levels in Prl cells from both FW- and SW-acclimated fish increased in direct proportion to increases in extracellular osmolality both\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Taken together, these results indicate that the osmosensitivity of the tilapia RPD is modulated by environmental salinity with respect to hormone release and gene expression.\n                  <\/jats:p>","DOI":"10.1530\/joe-11-0384","type":"journal-article","created":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T02:08:50Z","timestamp":1327025330000},"page":"89-98","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":38,"title":["Prolactin177, prolactin188 and prolactin receptor 2 in the pituitary of the euryhaline tilapia, Oreochromis mossambicus, are differentially osmosensitive"],"prefix":"10.1530","volume":"213","author":[{"given":"Andre P","family":"Seale","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin P","family":"Moorman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob J","family":"Stagg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason P","family":"Breves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren T","family":"Lerner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E Gordon","family":"Grau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"issue":"1","key":"1_10128293","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1006\/gcen.1993.1017","volume":"89","author":"Ayson","year":"1993","journal-title":"General and comparative endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0016-6480","issn-type":"print"},{"key":"2_25205474","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1002\/jez.1402640108","volume":"264","year":"1992","journal-title":"The Journal of experimental zoology","ISSN":"https:\/\/id.crossref.org\/issn\/0022-104X","issn-type":"print"},{"key":"3_39692893","first-page":"432","volume":"313","year":"2010","journal-title":"The Journal of experimental zoology","ISSN":"https:\/\/id.crossref.org\/issn\/0022-104X","issn-type":"print"},{"key":"4_39692894","first-page":"R702","volume":"299","year":"2010","journal-title":"American Journal of Physiology -- Legacy Content","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9513","issn-type":"print"},{"key":"5_42204825","first-page":"194","volume":"158","year":"2011","journal-title":"Comparative Biochemistry and Physiology. 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