{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:17Z","timestamp":1784557217409,"version":"3.55.0"},"reference-count":40,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>\n                    The anterior pituitary gland comprises five types of endocrine cells plus non-endocrine cells including folliculostellate cells, endothelial cells, and capillary mural cells (pericytes). In addition to being controlled by the hypothalamic\u2013pituitary\u2013target organ axis, the functions of these cells are likely regulated by local cell and extracellular matrix (ECM) interactions. However, these complex interactions are not fully understood. We investigated folliculostellate cell-mediated cell-to-cell interaction. Using S100\u03b2-GFP transgenic rats, which express GFP in folliculostellate cells, we designed a three-dimensional cell culture to examine the effects of folliculostellate cells. Interestingly, removal of folliculostellate cells reduced collagen synthesis (\n                    <jats:italic>Col1a1<\/jats:italic>\n                    and\n                    <jats:italic>Col3a1<\/jats:italic>\n                    ). Because pericytes are important collagen-producing cells in the gland, we stained for desmin (a pericyte marker). Removal of folliculostellate cells resulted in fewer desmin-positive pericytes and less desmin mRNA. We then attempted to identify the factor mediating folliculostellate cell\u2013pericyte interaction. RT-PCR and\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization revealed that the important profibrotic factor transforming growth factor beta-2 (TGF\u03b22) was specifically expressed in folliculostellate cells and that TGF\u03b2 receptor II was expressed in pericytes, endothelial cells, and parenchymal cells. Immunocytochemistry showed that TGF\u03b22 induced SMAD2 nuclear translocation in pericytes. TGF\u03b22 increased collagen synthesis in a dose-dependent manner. This action was completely blocked by TGF\u03b2 receptor I inhibitor (SB431542). Diminished collagen synthesis in folliculostellate cell-deficient cell aggregates was partially recovered by TGF\u03b22. TGF\u03b22-mediated folliculostellate cell\u2013pericyte interaction appears to be essential for collagen synthesis in rat anterior pituitary. This finding sheds new light on local cell\u2013ECM interactions in the gland.\n                  <\/jats:p>","DOI":"10.1530\/joe-16-0033","type":"journal-article","created":{"date-parts":[[2016,6,7]],"date-time":"2016-06-07T21:21:35Z","timestamp":1465334495000},"page":"159-170","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":15,"title":["Folliculostellate cell interacts with pericyte via TGF\u03b22 in rat anterior pituitary"],"prefix":"10.1530","volume":"229","author":[{"given":"Takehiro","family":"Tsukada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morio","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kotaro","family":"Horiguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Kouki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoshi","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yashiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2016060718191402000_229.2.159.1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.01949"},{"key":"2016060718191402000_229.2.159.2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000182903.16652.d7"},{"key":"2016060718191402000_229.2.159.3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1267\/ahc.15020","article-title":"Maintenance of the extracellular matrix in rat anterior pituitary gland: identification of cells expressing tissue inhibitors of metalloproteinases","volume":"48","author":"Azuma","year":"2015","journal-title":"Acta Histochemica et Cytochemica"},{"key":"2016060718191402000_229.2.159.4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00171.2013"},{"key":"2016060718191402000_229.2.159.5","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.3.1444"},{"key":"2016060718191402000_229.2.159.6","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1490019"},{"key":"2016060718191402000_229.2.159.7","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0345-7"},{"key":"2016060718191402000_229.2.159.8","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s00441-007-0423-5","article-title":"Expression of retinaldehyde dehydrogenase 1 in the anterior pituitary glands of adult rats.","volume":"329","author":"Fujiwara","year":"2007","journal-title":"Cell and Tissue Research"},{"key":"2016060718191402000_229.2.159.9","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s00441-010-1078-1","article-title":"In situ hybridization reveals that type I and III collagens are produced by pericytes in the anterior pituitary gland of rats","volume":"342","author":"Fujiwara","year":"2010","journal-title":"Cell and Tissue Research"},{"key":"2016060718191402000_229.2.159.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2003.08.002"},{"key":"2016060718191402000_229.2.159.11","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/jor.20997","article-title":"Expression patterns of collagen types I and III in the capsule of a rat knee contracture model","volume":"28","author":"Hagiwara","year":"2010","journal-title":"Journal of Orthopaedic Research"},{"key":"2016060718191402000_229.2.159.12","doi-asserted-by":"publisher","DOI":"10.1038\/nature06868"},{"key":"2016060718191402000_229.2.159.13","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.4.1528"},{"key":"2016060718191402000_229.2.159.14","first-page":"481","article-title":"Laminin in the anterior pituitary gland of the rat. 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