{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:24:46Z","timestamp":1789381486207,"version":"build-2803163510"},"reference-count":62,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:p>\n                    Our previous study showed that the\n                    <jats:italic>in vivo<\/jats:italic>\n                    positive effects of 17\u03b1,20\u03b2-dihydroxy-4-pregnen-3-one (DHP), the major progestin in zebrafish, on early spermatogenesis was much stronger than the\n                    <jats:italic>ex vivo<\/jats:italic>\n                    ones, which may suggest an effect of DHP on the expression of gonadotropins. In our present study, we first observed that\n                    <jats:italic>fshb<\/jats:italic>\n                    and\n                    <jats:italic>lhb<\/jats:italic>\n                    mRNA levels in the pituitary of male adult zebrafish were greatly inhibited by 3 weeks exposure to 10nM estradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ). However, an additional 24h 100nM DHP exposure not only reversed the E\n                    <jats:sub>2<\/jats:sub>\n                    -induced inhibition, but also significantly increased the expression of\n                    <jats:italic>fshb<\/jats:italic>\n                    and\n                    <jats:italic>lhb<\/jats:italic>\n                    mRNA. These stimulatory effects were also observed in male adult fish without E\n                    <jats:sub>2<\/jats:sub>\n                    pretreatment, and a time course experiment showed that it took 24h for\n                    <jats:italic>fshb<\/jats:italic>\n                    and 12h for\n                    <jats:italic>lhb<\/jats:italic>\n                    to respond significantly. Because these stimulatory activities were partially antagonized by a nuclear progesterone receptor (Pgr) antagonist mifepristone, we generated a Pgr-knockout (\n                    <jats:italic>pgr<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    ) model using the TALEN technique. With and without DHP\n                    <jats:italic>in vivo<\/jats:italic>\n                    treatment,\n                    <jats:italic>fshb<\/jats:italic>\n                    and\n                    <jats:italic>lhb<\/jats:italic>\n                    mRNA levels of\n                    <jats:italic>pgr<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    were significantly lower than those of\n                    <jats:italic>pgr<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    . Furthermore,\n                    <jats:italic>ex vivo<\/jats:italic>\n                    treatment of pituitary fragments of\n                    <jats:italic>pgr<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    with DHP stimulated\n                    <jats:italic>lhb<\/jats:italic>\n                    , but not\n                    <jats:italic>fshb<\/jats:italic>\n                    mRNA expression. Results from double-colored fluorescent\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization showed that\n                    <jats:italic>pgr<\/jats:italic>\n                    mRNA was expressed only in\n                    <jats:italic>fshb<\/jats:italic>\n                    -expressing cells. Taken together, our results indicated that DHP participated in the regulation of neuroendocrine control of reproduction in male zebrafish, and exerted a Pgr-mediated direct stimulatory effect on\n                    <jats:italic>fshb<\/jats:italic>\n                    mRNA at pituitary level.\n                  <\/jats:p>","DOI":"10.1530\/joe-16-0073","type":"journal-article","created":{"date-parts":[[2016,4,25]],"date-time":"2016-04-25T23:53:32Z","timestamp":1461628412000},"page":"143-156","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":19,"title":["Progestin increases the expression of gonadotropins in pituitaries of male zebrafish"],"prefix":"10.1530","volume":"230","author":[{"given":"Cuili","family":"Wang","sequence":"first","affiliation":[{"name":"1State Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China"},{"name":"2Fujian Collaborative Innovation Center 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanshu","family":"Hong","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China"},{"name":"2Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamen, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhu","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, China"},{"name":"4Department of BiologyEast Carolina University, Greenville, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi X","family":"Chen","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Marine Environmental ScienceCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, 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