{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:54Z","timestamp":1784557194827,"version":"3.55.0"},"reference-count":41,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>\n                    Cancer cachexia (CC) is a progressive metabolic syndrome that is marked by severe body weight loss. Metabolic disarrangement of fat tissues is a very early event in CC, followed by adipose tissue (AT) atrophy and remodelling. However, there is little information regarding the possible involvement of cellular turnover in this process. Thus, in this study, we evaluated the effect of CC on AT turnover and fibrosis of mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue depots as possible factors that contribute to AT atrophy. CC was induced by a subcutaneous injection of Walker tumour cells (2\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    ) in Wistar rats, and control animals received only saline. The experimental rats were randomly divided into four experimental groups: 0 days, 4 days, 7 days and 14 days after injection. AT turnover was analysed according to the\n                    <jats:italic>Pref1\/Adiponectin<\/jats:italic>\n                    ratio of gene expression from the stromal vascular fraction and pro-apoptotic CASPASE3 and CASPASE9 from MEAT and RPAT. Fibrosis was verified according to the total collagen levels and expression of extracellular matrix genes. AT turnover was verified by measurements of lipolytic protein expression. We found that the\n                    <jats:italic>Pref1\/Adiponectin<\/jats:italic>\n                    ratio was decreased in RPAT (81.85%,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05) with no changes in MEAT compared with the respective controls. CASPASE3 and CASPASE9 were activated on day 14 only in RPAT. Collagen was increased on day 7 in RPAT (127%) and MEAT (4.3-fold). The\n                    <jats:italic>Collagen1A1<\/jats:italic>\n                    ,\n                    <jats:italic>Collagen3A1<\/jats:italic>\n                    ,\n                    <jats:italic>Mmp2<\/jats:italic>\n                    and\n                    <jats:italic>Mmp9<\/jats:italic>\n                    mRNA levels were upregulated only in MEAT in CC. Lipid turnover was verified in RPAT and was not modified in CC. We concluded that the results suggest that CC affects RPAT cellular turnover, which may be determinant for RPAT atrophy.\n                  <\/jats:p>","DOI":"10.1530\/joe-16-0305","type":"journal-article","created":{"date-parts":[[2017,1,4]],"date-time":"2017-01-04T21:10:45Z","timestamp":1483564245000},"page":"493-500","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":18,"title":["Cancer cachexia differentially regulates visceral adipose tissue turnover"],"prefix":"10.1530","volume":"232","author":[{"given":"Felipe de Oliveira","family":"Franco","sequence":"first","affiliation":[{"name":"1Laboratory of Adipose Tissue BiologyCenter for Integrated Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magno Alves","family":"Lopes","sequence":"additional","affiliation":[{"name":"1Laboratory of Adipose Tissue BiologyCenter for Integrated Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe dos Santos","family":"Henriques","sequence":"additional","affiliation":[{"name":"1Laboratory of Adipose Tissue BiologyCenter for Integrated Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Xavier das","family":"Neves","sequence":"additional","affiliation":[{"name":"2Cancer Metabolism Research GroupInstitute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ces\u00e1rio","family":"Bianchi Filho","sequence":"additional","affiliation":[{"name":"3Center for Clinical and Translational ResearchUniversity of Mogi das Cruzes, Mogi das Cruzes, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel 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