{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:48Z","timestamp":1784557248102,"version":"3.55.0"},"reference-count":50,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>\n                    In mammals, pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic hormone with diverse functions but its role in prolactin (PRL) regulation is highly controversial. To shed light on Prl regulation by PACAP in fish model, grass carp pituitary cells was used as a model to examine the receptor specificity and signal transduction for PACAP modulation of\n                    <jats:italic>prl<\/jats:italic>\n                    gene expression in the carp pituitary. Using RT-PCR, PACAP-selective PAC1 receptor was detected in carp lactotrophs. In carp pituitary cells, nanomolar doses of PACAP, but not VIP, could elevate Prl secretion and protein production with concurrent rise in\n                    <jats:italic>prl<\/jats:italic>\n                    mRNA and these stimulatory effects were blocked by PACAP antagonist but not VIP antagonist. PACAP-induced\n                    <jats:italic>prl<\/jats:italic>\n                    mRNA expression could be mimicked by activating adenylate cyclase (AC), increasing cAMP level by cAMP analog, or increasing intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]i) by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ionophore\/voltage-sensitive Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel (VSCC) activator. PACAP-induced\n                    <jats:italic>prl<\/jats:italic>\n                    gene expression, however, was attenuated\/abolished by suppressing cAMP production, inhibiting PKA activity, blocking [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]i mobilization and VSCC activation, calmodulin (CaM) antagonism, and inactivation of JNK and CaM Kinase II (CaMK-II). Similar sensitivity to CaM, JNK, and CaMK-II blockade was also noted by substituting cAMP analog for PACAP as the stimulant for\n                    <jats:italic>prl<\/jats:italic>\n                    mRNA expression. These results, as a whole, provide evidence for the first time that (i) PACAP activation of PAC1 receptor expressed in carp lactotrophs could induce Prl synthesis and secretion, and (ii) Prl production induced by PACAP was mediated by upregulation of\n                    <jats:italic>prl<\/jats:italic>\n                    gene expression, presumably via functional coupling of cAMP\/PKA-, Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \/CaM-, and MAPK-dependent cascades.\n                  <\/jats:p>","DOI":"10.1530\/joe-16-0433","type":"journal-article","created":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T21:10:43Z","timestamp":1485551443000},"page":"37-51","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":9,"title":["Mechanisms for PACAP-induced prolactin gene expression in grass carp pituitary 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