{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:35Z","timestamp":1784557235430,"version":"3.55.0"},"reference-count":52,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>Poor nutrition plays a fundamental role in the development of insulin resistance, an underlying characteristic of type 2 diabetes. We have previously shown that high-fat diet-induced insulin resistance in rats can be ameliorated by a single glucose meal, but the mechanisms for this observation remain unresolved. To determine if this phenomenon is mediated by gut or hepatoportal factors, male Wistar rats were fed a high-fat diet for 3 weeks before receiving one of five interventions: high-fat meal, glucose gavage, high-glucose meal, systemic glucose infusion or portal glucose infusion. Insulin sensitivity was assessed the following day in conscious animals by a hyperinsulinaemic-euglycaemic clamp. An oral glucose load consistently improved insulin sensitivity in high-fat-fed rats, establishing the reproducibility of this model. A systemic infusion of a glucose load did not affect insulin sensitivity, indicating that the physiological response to oral glucose was not due solely to increased glucose turnover or withdrawal of dietary lipid. A portal infusion of glucose produced the largest improvement in insulin sensitivity, implicating a role for the hepatoportal region rather than the gastrointestinal tract in mediating the effect of glucose to improve lipid-induced insulin resistance. These results further deepen our understanding of the mechanism of glucose-mediated regulation of insulin sensitivity and provide new insight into the role of nutrition in whole body metabolism.<\/jats:p>","DOI":"10.1530\/joe-18-0566","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T11:39:03Z","timestamp":1554205143000},"page":"189-199","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":5,"title":["Glucose mediates insulin sensitivity via a hepatoportal mechanism in high-fat-fed rats"],"prefix":"10.1530","volume":"241","author":[{"given":"Holly M","family":"Johnson","sequence":"first","affiliation":[{"name":"1The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Stanfield","sequence":"additional","affiliation":[{"name":"1The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace J","family":"Campbell","sequence":"additional","affiliation":[{"name":"1The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica E","family":"Eberl","sequence":"additional","affiliation":[{"name":"1The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J","family":"Cooney","sequence":"additional","affiliation":[{"name":"2The University of Sydney, Charles Perkins Centre, School of Medical Sciences, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim S","family":"Bell-Anderson","sequence":"additional","affiliation":[{"name":"1The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"ref521","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1001\/archinte.1927.00130120077005","article-title":"Dietary factors that influence the dextrose tolerance test","volume":"40","year":"1927","journal-title":"Archives of Internal Medicine"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1079\/NRR2005109","article-title":"Determining the relationship between dietary carbohydrate intake and insulin resistance","volume":"18","year":"2005","journal-title":"Nutrition Research Reviews"},{"key":"ref11","first-page":"431","article-title":"An anterograde tracing study of the vagal innervation of rat liver, portal vein and biliary system","volume":"186","year":"1992","journal-title":"Anatomy and Embryology"},{"key":"ref161","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1530\/EJE-06-0740","article-title":"Metabolic and hormonal changes during the refeeding period of prolonged fasting","volume":"157","year":"2007","journal-title":"European Journal of Endocrinology"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1079\/NRR2005109","article-title":"Determining the relationship between dietary carbohydrate intake and insulin resistance","volume":"18","year":"2005","journal-title":"Nutrition Research Reviews"},{"key":"ref221","first-page":"314 E251","article-title":"Modeling insulin resistance in rodents by alterations in diet: what have high-fat and high-calorie diets revealed?","volume":"314","year":"2018","journal-title":"American Journal of Physiology. 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