{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:47Z","timestamp":1784557247212,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,9]]},"abstract":"\n Hyperpolarized [1-\n 13<\/jats:sup>\n C]pyruvate magnetic resonance (MR) spectroscopy has the unique ability to detect real-time metabolic changes\n in vivo<\/jats:italic>\n owing to its high sensitivity compared with thermal MR and high specificity compared with other metabolic imaging methods. The aim of this study was to explore the potential of hyperpolarized MR spectroscopy for quantification of liver pyruvate metabolism during a hyperinsulinemic\u2013isoglycemic clamp in mice. Hyperpolarized [1-\n 13<\/jats:sup>\n C]pyruvate was used for\n in vivo<\/jats:italic>\n MR spectroscopy of liver pyruvate metabolism in mice. Mice were divided into two groups: (i) non-stimulated 5-h fasted mice and (ii) hyperinsulinemic-isoglycemic clamped mice. During clamp conditions, insulin and donor blood were administered at a constant rate, whereas glucose was infused to maintain isoglycemia. When steady state was reached, insulin-stimulated mice were rapidly infused with hyperpolarized [1-\n 13<\/jats:sup>\n C]pyruvate for real-time tracking of the dynamic distribution of metabolic derivatives from pyruvate, such as [1-\n 13<\/jats:sup>\n C]lactate, [1-\n 13<\/jats:sup>\n C]alanine and [\n 13<\/jats:sup>\n C]bicarbonate. Isotopomer analysis of plasma glucose confirmed\n 13<\/jats:sup>\n C-incorporation from [1-\n 13<\/jats:sup>\n C]pyruvate into glucose was increased in fasted mice compared with insulin-stimulated mice, demonstrating an increased gluconeogenesis in fasted mice. The AUC ratios for [1-\n 13<\/jats:sup>\n C]alanine\/[1-\n 13<\/jats:sup>\n C]pyruvate (38.2%), [1-\n 13<\/jats:sup>\n C]lactate\/[1-\n 13<\/jats:sup>\n C]pyruvate (41.8%) and [\n 13<\/jats:sup>\n C]bicarbonate\/[1-\n 13<\/jats:sup>\n C]pyruvate (169%) all increased significantly during insulin stimulation. Hyperpolarized [1-\n 13<\/jats:sup>\n C]pyruvate can be used for\n in vivo<\/jats:italic>\n MR spectroscopy of liver pyruvate metabolism during hyperinsulinemic-isoglycemic clamp conditions. Under these conditions, insulin decreased gluconeogenesis and increased [1-\n 13<\/jats:sup>\n C]alanine, [1-\n 13<\/jats:sup>\n C]lactate and [\n 13<\/jats:sup>\n C]bicarbonate after a [1-\n 13<\/jats:sup>\n C]pyruvate bolus. This application of\n in vivo<\/jats:italic>\n spectroscopy has the potential to identify impairments in specific metabolic pathways in the liver associated with obesity, insulin resistance and nonalcoholic fatty liver disease.\n <\/jats:p>","DOI":"10.1530\/joe-19-0159","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T10:07:06Z","timestamp":1563271626000},"page":"251-260","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of mouse liver [1-13C]pyruvate metabolism by dynamic hyperpolarized MRS"],"prefix":"10.1530","volume":"242","author":[{"given":"Kasper","family":"Faarkrog H\u00f8yer","sequence":"first","affiliation":[{"name":"1Department of Clinical Medicine, The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"},{"name":"2Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoffer","family":"Laustsen","sequence":"additional","affiliation":[{"name":"1Department of Clinical Medicine, The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Ringgaard","sequence":"additional","affiliation":[{"name":"1Department of Clinical Medicine, The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyun","family":"Qi","sequence":"additional","affiliation":[{"name":"1Department of Clinical Medicine, The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian \u00d8stergaard","family":"Mariager","sequence":"additional","affiliation":[{"name":"1Department of Clinical Medicine, The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Svava","family":"Nielsen","sequence":"additional","affiliation":[{"name":"2Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrik Kr\u00e6mer","family":"Sundekilde","sequence":"additional","affiliation":[{"name":"3Department of Food Science, Aarhus University, Aarslev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas T","family":"Treebak","sequence":"additional","affiliation":[{"name":"2Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Jessen","sequence":"additional","affiliation":[{"name":"4Department of Biomedicine, Research Laboratory for Biochemical Pathology, Aarhus University, Aarhus, Denmark"},{"name":"5Department of Clinical Pharmacology, Aarhus University Hospital, Aarhus, Denmark"},{"name":"6Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"St\u00f8dkilde-J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"1Department of Clinical Medicine, The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/242\/3\/JOE-19-0159.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/242\/3\/JOE-19-0159.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:47Z","timestamp":1784555807000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/242\/3\/251-260\/5156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/joe-19-0159","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9]]}}}