{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:28:40Z","timestamp":1791664120308,"version":"4.3.4"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>\n                    Adenosine 2A receptor (A\n                    <jats:sub>2A<\/jats:sub>\n                    R) exerts a protective role in obesity-related non-alcoholic fatty liver disease. Here, we examined whether A\n                    <jats:sub>2A<\/jats:sub>\n                    R protects against non-alcoholic steatohepatitis (NASH). In C57BL\/6J mice, feeding a methionine- and choline-deficient diet (MCD) resulted in significant weight loss, overt hepatic steatosis, and massive aggregation of macrophages in the liver compared with mice fed a chow diet. MCD feeding also significantly increased the numbers of A\n                    <jats:sub>2A<\/jats:sub>\n                    R-positive macrophages\/Kupffer cells in liver sections although decreasing A\n                    <jats:sub>2A<\/jats:sub>\n                    R amount in liver lysates compared with chow diet feeding. Next, MCD-induced NASH phenotype was examined in A\n                    <jats:sub>2A<\/jats:sub>\n                    R-disrupted mice and control mice. Upon MCD feeding, A\n                    <jats:sub>2A<\/jats:sub>\n                    R-disruptd mice and control mice displayed comparable decreases in body weight and fat mass. However, MCD-fed A\n                    <jats:sub>2A<\/jats:sub>\n                    R-disrupted mice revealed greater liver weight and increased severity of hepatic steatosis compared with MCD-fed control mice. Moreover, A\n                    <jats:sub>2A<\/jats:sub>\n                    R-disupted mice displayed increased severity of MCD-induced liver inflammation, indicated by massive aggregation of macrophages and increased phosphorylation states of Jun-N terminal kinase (JNK) p46 and nuclear factor kappa B (NF\u03baB) p65 and mRNA levels of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , incubation with MCD-mimicking media increased lipopolysaccharide (LPS)-induced phosphorylation states of JNK p46 and\/or NF\u03baB p65 and cytokine mRNAs in control macrophages and RAW264.7 cells, but not primary hepatocytes. Additionally, MCD-mimicking media significantly increased lipopolysaccharide-induced phosphorylation states of p38 and NF\u03baB p65 in A\n                    <jats:sub>2A<\/jats:sub>\n                    R-deficient macrophages, but insignificantly decreased lipopolysaccharide-induced phosphorylation states of JNK p46 and NF\u03baB p65 in A\n                    <jats:sub>2A<\/jats:sub>\n                    R-deficient hepatocytes. Collectively, these results suggest that A\n                    <jats:sub>2A<\/jats:sub>\n                    R disruption exacerbates MCD-induced NASH, which is attributable to, in large part, increased inflammatory responses in macrophages.\n                  <\/jats:p>","DOI":"10.1530\/joe-19-0198","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T08:15:26Z","timestamp":1568362526000},"page":"199-209","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":16,"title":["Mice lacking adenosine 2A receptor reveal increased severity of MCD-induced NASH"],"prefix":"10.1530","volume":"243","author":[{"given":"Jing","family":"Zhou","sequence":"first","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honggui","family":"Li","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuli","family":"Cai","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"},{"name":"2Department of Endocrinology, Renmin Hospital, Wuhan University, Wuhan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linqiang","family":"Ma","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"},{"name":"3Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China"},{"name":"4The Laboratory of Lipid & Glucose Metabolism, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Destiny","family":"Matthews","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bangchao","family":"Lu","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"},{"name":"5Department of Geriatrics, Nanjing Drum Tower Hospital, the Affiliated Nanjing Hospital of Nanjing University Medical School, Nanjing, Jiangshu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilian","family":"Zhu","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"},{"name":"6Department of Endocrinology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanming","family":"Chen","sequence":"additional","affiliation":[{"name":"6Department of Endocrinology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxian","family":"Qian","sequence":"additional","affiliation":[{"name":"7Department of Cardiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiu","family":"Xiao","sequence":"additional","affiliation":[{"name":"4The Laboratory of Lipid & Glucose Metabolism, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qifu","family":"Li","sequence":"additional","affiliation":[{"name":"3Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaodong","family":"Guo","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Huo","sequence":"additional","affiliation":[{"name":"8Vascular Biology Center, Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhao","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"},{"name":"9Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Tian","sequence":"additional","affiliation":[{"name":"10Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingsheng","family":"Li","sequence":"additional","affiliation":[{"name":"11Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaodong","family":"Wu","sequence":"additional","affiliation":[{"name":"1Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/243\/3\/JOE-19-0198.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/243\/3\/JOE-19-0198.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:49Z","timestamp":1784555809000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/243\/3\/199-209\/5198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/joe-19-0198","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12]]}}}