{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:13:43Z","timestamp":1787022823843,"version":"build-2736575974"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,8]]},"abstract":"\n Adrenalectomy (ADX) induces hypophagia and glucocorticoids counter-regulate the peripheral metabolic effects of insulin. This study evaluated the effects of ADX on ICV (lateral ventricle) injection of insulin-induced changes on food intake, mRNA expression of hypothalamic neuropeptides (insulin receptor (\n InsR<\/jats:italic>\n ), proopiomelanocortin, cocaine and amphetamine-regulated transcript (\n Cart<\/jats:italic>\n ), agouti-related protein, neuropeptide Y (\n Npy<\/jats:italic>\n ) in the arcuate nucleus of the hypothalamus (ARC), corticotrophin-releasing factor in the paraventricular nucleus of the hypothalamus) and hypothalamic protein content of insulin signaling-related molecules (insulin receptor substrate (IRS) 1, protein kinase B (AKT), extracellular-signal-regulated kinase (ERK1\/2), c-Jun N-terminal kinase (JNK), protein tyrosine phosphatase-1B (PTP1B) and T cell protein tyrosine phosphatase (TCPTP)) Compared with sham animals, ADX increased the hypothalamic content of pJNK\/JNK, PTP1B and TCPTP, as well as decreased mRNA expression of\n InsR<\/jats:italic>\n , and corticosterone (B) treatment reversed these effects. Insulin central injection enhanced hypothalamic content of pAKT\/AKT and\n Cart<\/jats:italic>\n mRNA expression, decreased\n Npy<\/jats:italic>\n mRNA expression and food intake only in sham rats, without effects in ADX and ADX\u2009+\u2009B rats. Insulin did not alter the hypothalamic phosphorylation of IRS1 and ERK1\/2 in the three experimental groups. These data demonstrate that ADX reduces the expression of\n InsR<\/jats:italic>\n and increases insulin counter-regulators in the hypothalamus, as well as ADX abolishes hypophagia, activation of hypothalamic AKT pathway and changes in\n Cart<\/jats:italic>\n and\n Npy<\/jats:italic>\n mRNA expression in the ARC induced by insulin. Thus, the higher levels of insulin counter-regulatory proteins and lower expression of\n InsR<\/jats:italic>\n in the hypothalamus are likely to underlie impaired insulin-induced hypophagia and responses in the hypothalamus after ADX.\n <\/jats:p>","DOI":"10.1530\/joe-19-0217","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T12:57:55Z","timestamp":1560171475000},"page":"125-138","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":2,"title":["Adrenalectomy impairs insulin-induced hypophagia and related hypothalamic changes"],"prefix":"10.1530","volume":"242","author":[{"given":"Ernane Torres","family":"Uchoa","sequence":"first","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"},{"name":"2Department of Physiological Sciences, State University of Londrina, Londrina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Beatriz","family":"Marangon","sequence":"additional","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Rorato","sequence":"additional","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"},{"name":"3Biotechnology Unit, University of Ribeirao Preto, Ribeirao Preto, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia Graciela","family":"Ruginsk","sequence":"additional","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"},{"name":"4Department of Physiological Sciences, Biomedical Sciences Institute, Federal University of Alfenas, Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Kniess","family":"Debarba","sequence":"additional","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Antunes-Rodrigues","sequence":"additional","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucila L K","family":"Elias","sequence":"additional","affiliation":[{"name":"1Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/242\/2\/JOE-19-0217.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/242\/2\/JOE-19-0217.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:50Z","timestamp":1784555810000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/242\/2\/125-138\/5145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/joe-19-0217","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8]]}}}