{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:29:00Z","timestamp":1790879340914,"version":"4.1.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1]]},"abstract":"\n Changes in zebrafish testicular gene expression induced by follicle-stimulating hormone (Fsh) or anti-Mullerian hormone (Amh) suggested that Amh inhibition and Fsh stimulation of spermatogenesis involved up and downregulation, respectively, of prostaglandin (PG) signaling. We found that Sertoli cells contacting type A undifferentiated (A\n und<\/jats:sub>\n ) and differentiating (A\n diff<\/jats:sub>\n ) spermatogonia expressed a key enzyme of PG production (Ptgs2); previous work showed that Sertoli cells contacting A\n diff<\/jats:sub>\n and B spermatogonia and spermatocytes showed\n ptges3b<\/jats:italic>\n expression, an enzyme catalyzing PGE\n 2<\/jats:sub>\n production. In primary testis tissue cultures, PGE\n 2<\/jats:sub>\n , but not PGD\n 2<\/jats:sub>\n or PGF\n 2\u03b1<\/jats:sub>\n , reduced the mitotic activity of A\n diff<\/jats:sub>\n and their development into B spermatogonia.\n Vice versa<\/jats:italic>\n , inhibiting PG production increased the mitotic activity of A\n diff<\/jats:sub>\n and B spermatogonia. Studies with pharmacological PG receptor antagonists suggest that an Ep4 receptor mediates the inhibitory effects on the development of spermatogonia, and cell-sorting experiments indicated this receptor is expressed mainly by testicular somatic cells. Combined inhibition of PG and steroid production moreover reduced the mitotic activity of A\n und<\/jats:sub>\n spermatogonia and led to their partial depletion, suggesting that androgens (and\/or other testicular steroids), supported by PGE\n 2<\/jats:sub>\n , otherwise prevent depletion of A\n und<\/jats:sub>\n . Androgens also decreased testicular PGE\n 2<\/jats:sub>\n production, increased the transcript levels of the enzyme-catabolizing PGs and decreased PGE\n 2<\/jats:sub>\n receptor\n ptger4b<\/jats:italic>\n transcript levels. Also Fsh potentially reduced, independent of androgens, PGE\n 2<\/jats:sub>\n production by decreasing\n ptges3b<\/jats:italic>\n transcript levels. Taken together, our results indicate that PGE\n 2<\/jats:sub>\n , via Ep4 receptors, favors self-renewal in conjunction with androgens and, independent of Fsh and androgens, inhibits differentiating divisions of spermatogonia.\n <\/jats:p>","DOI":"10.1530\/joe-19-0309","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T09:05:55Z","timestamp":1570698355000},"page":"163-175","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":27,"title":["PGE2 inhibits spermatogonia differentiation in zebrafish: interaction with Fsh and an androgen"],"prefix":"10.1530","volume":"244","author":[{"given":"Diego","family":"Crespo","sequence":"first","affiliation":[{"name":"1Reproductive Biology Group, Division Developmental Biology, Department Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moline Severino","family":"Lemos","sequence":"additional","affiliation":[{"name":"2Laboratory of Cell Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"3State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Fujian, People\u2019s Republic of China"},{"name":"4Institute of Oceanography, Minjiang University, Fuzhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Safian","sequence":"additional","affiliation":[{"name":"1Reproductive Biology Group, Division Developmental Biology, Department Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"Norberg","sequence":"additional","affiliation":[{"name":"5Institute of Marine Research, Austevoll Research Station, Storeb\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Bogerd","sequence":"additional","affiliation":[{"name":"1Reproductive Biology Group, Division Developmental Biology, Department Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00fcdiger W","family":"Schulz","sequence":"additional","affiliation":[{"name":"1Reproductive Biology Group, Division Developmental Biology, Department Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands"},{"name":"6Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/244\/1\/JOE-19-0309.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/244\/1\/JOE-19-0309.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:55Z","timestamp":1784555815000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/244\/1\/163-175\/5209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/joe-19-0309","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]}}}