{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:51Z","timestamp":1784557251546,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>Pituitary-directed medical treatment for Cushing\u2019s disease (CD) is currently represented by membrane receptor targeting drugs (somatostatin analogs and dopamine agonists). Somatostatin and dopamine receptors are regulated by \u03b2-arrestins, which have been shown to be differentially regulated by glucocorticoids in non-neuroendocrine cells. In this study we investigated the effects of glucocorticoids on \u03b2-arrestin expression in corticotroph tumor cells. First, AtT20 cells, a mouse model of CD, were exposed to dexamethasone (Dex) at different time points and \u03b2-arrestin expression was evaluated at mRNA and protein levels. Futhermore, \u03b2-arrestin mRNA expression was evaluated in 17 human corticotroph adenoma samples and correlated to patients\u2019 pre-operative cortisol levels. We observed that Dex treatment induced a time-dependent increase in \u03b2-arrestin 1 mRNA expression and a decrease in \u03b2-arrestin 2. The same modulation pattern was observed at protein level. Dex-mediated modulation of \u03b2-arrestins was abolished by co-treatment with mifepristone, and Dex withdrawal restored \u03b2-arrestin expression to basal levels after 72 h. The evaluation of \u03b2-arrestin mRNA in corticotroph adenomas from CD patients with variable disease activity showed a significant positive correlation between \u03b2-arrestin 1 mRNA and urinary cortisol levels. The effect of glucocorticoids on \u03b2-arrestin levels was confirmed by the analysis of two samples from a single patient, which underwent adenomectomy twice, with different pre-operative cortisol levels. In conclusion, glucocorticoids induce an inverse modulation of the two \u03b2-arrestin isofoms in corticotroph tumor cells. Since \u03b2-arrestins regulate membrane receptor functions, this finding may help to better understand the variable response to pituitary-targeting drugs in patients with Cushing\u2019s disease.<\/jats:p>","DOI":"10.1530\/joe-19-0311","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T12:54:25Z","timestamp":1580993665000},"page":"101-113","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":4,"title":["\u03b2-arrestin expression in corticotroph tumor cells is modulated by glucocorticoids"],"prefix":"10.1530","volume":"245","author":[{"given":"Federico","family":"Gatto","sequence":"first","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"},{"name":"2Endocrinology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A","family":"Feelders","sequence":"additional","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"},{"name":"3Pituitary Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"van der Pas","sequence":"additional","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"van Koetsveld","sequence":"additional","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Bruzzone","sequence":"additional","affiliation":[{"name":"4Department of Internal Medicine and & Medical Specialties (DIMI) and Center of Excellence for Biomedical Research (CEBR), University of Genoa, Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marica","family":"Arvigo","sequence":"additional","affiliation":[{"name":"4Department of Internal Medicine and & Medical Specialties (DIMI) and Center of Excellence for Biomedical Research (CEBR), University of Genoa, Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadime","family":"Dogan","sequence":"additional","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Lamberts","sequence":"additional","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Ferone","sequence":"additional","affiliation":[{"name":"2Endocrinology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy"},{"name":"4Department of Internal Medicine and & Medical Specialties (DIMI) and Center of Excellence for Biomedical Research (CEBR), University of Genoa, Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Hofland","sequence":"additional","affiliation":[{"name":"1Department of Internal Medicine, Rotterdam, The Netherlands"},{"name":"3Pituitary Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/245\/1\/JOE-19-0311.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/245\/1\/JOE-19-0311.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:55Z","timestamp":1784555815000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/245\/1\/101-113\/5343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/joe-19-0311","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4]]}}}