{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:21:11Z","timestamp":1784557271738,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>\n                    MicroRNA-7 (miR-7) is an important modulator of a plenty of gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or\/and a hormone secreted excessively under stress, affects the synthesis and secretion of various hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key hormones which regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affects the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or \u03b2-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of \u03b2-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (\u03b22AR) and the RAF\/MEK\/ERK1\/2 signaling pathway. The phosphorylation of ERK1\/2 upregulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (\n                    <jats:italic>GLG1<\/jats:italic>\n                    ). These suggest that miR-7 mediates NE\u2019s effect on promoting FSH and LH synthesis in porcine pituitary.\n                  <\/jats:p>","DOI":"10.1530\/joe-19-0331","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T12:10:57Z","timestamp":1578312657000},"page":"459-471","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":14,"title":["miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary"],"prefix":"10.1530","volume":"244","author":[{"given":"Xin","family":"Li","sequence":"first","affiliation":[{"name":"1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjiao","family":"Li","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People\u2019s Republic of China"},{"name":"2College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"2College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojin","family":"Xu","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglin","family":"Zhang","sequence":"additional","affiliation":[{"name":"3Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Cui","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People\u2019s Republic of China"},{"name":"2College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People\u2019s Republic of China"},{"name":"3Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, People\u2019s Republic of China"},{"name":"4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/244\/3\/JOE-19-0331.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/244\/3\/JOE-19-0331.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:55Z","timestamp":1784555815000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/244\/3\/459-471\/5277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/joe-19-0331","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3]]}}}