{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:13:41Z","timestamp":1787022821992,"version":"build-2736575974"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1]]},"abstract":"\n Both estrogen and hydrogen sulfide (H\n 2<\/jats:sub>\n S) inhibit the proliferation of vascular smooth muscle cells (SMCs) and development of atherosclerosis. In the absence of endogenous H\n 2<\/jats:sub>\n S as occurred in CSE-knockout (KO) mouse, however, estrogen stimulates the proliferation of vascular SMCs. The underlying mechanisms for this seemingly controversial vascular effect of estrogen are unclear. In the present study, we demonstrated that the stimulatory effect of estrogen on the proliferation of CSE-KO SMCs was suppressed by the inhibitor of insulin-like growth factor-1 receptor (IGF-1R) or knockdown of IGF-1R protein expression. Estrogen downregulated the expression of insulin-like growth factor-1 (IGF-1) and IGF-1R in aortic tissues or aortic SMCs isolated from WT and CSE-KO mice. Furthermore, endogenous H\n 2<\/jats:sub>\n S downregulated IGF-1R, but upregulated estrogen receptor (ER)-\u03b1, in aortic tissues or SMCs. ER-\u03b1 and IGF-1R were co-located in SMCs and co-immunoprecipitated, which was decreased by H\n 2<\/jats:sub>\n S. Finally, both endogenous and exogenous H\n 2<\/jats:sub>\n S induced the S-sulfhydration of IGF-1R, but not ER-\u03b1, in WT-SMCs and CSE-KO SMCs, which underlies the decreased formation of IGF-1R\/ER-\u03b1 hybrid in the presence of H\n 2<\/jats:sub>\n S. Thus, the absence of H\n 2<\/jats:sub>\n S favors the interaction of estrogen with IGF-1R\/ER-\u03b1 hybrid to stimulate SMCs proliferation. The appreciation of a critical role of H\n 2<\/jats:sub>\n S in preventing estrogen-induced SMCs proliferation will help better understand the regulation of complex vascular effects of estrogen and sex-related cardiovascular diseases.\n <\/jats:p>","DOI":"10.1530\/joe-20-0190","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T18:52:39Z","timestamp":1603219959000},"page":"17-30","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":21,"title":["Interaction among estrogen, IGF-1, and H2S on smooth muscle cell proliferation"],"prefix":"10.1530","volume":"248","author":[{"given":"Tian","family":"Shuang","sequence":"first","affiliation":[{"name":"1Cardiovascular and Metabolic Research Unit, Laurentian University, Ontario, Canada"},{"name":"2School of Human Kinetics, Faculty of Health, Laurentian University, Ontario, Canada"},{"name":"3Department of Biology, Laurentian University, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Fu","sequence":"additional","affiliation":[{"name":"1Cardiovascular and Metabolic Research Unit, Laurentian University, Ontario, Canada"},{"name":"2School of Human Kinetics, Faculty of Health, Laurentian University, Ontario, Canada"},{"name":"4Health Sciences North Research Institute, Sudbury, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangdong","family":"Yang","sequence":"additional","affiliation":[{"name":"1Cardiovascular and Metabolic Research Unit, Laurentian University, Ontario, Canada"},{"name":"5Department of Chemistry and Biochemistry, Laurentian University, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"1Cardiovascular and Metabolic Research Unit, Laurentian University, Ontario, Canada"},{"name":"2School of Human Kinetics, Faculty of Health, Laurentian University, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongming","family":"Qian","sequence":"additional","affiliation":[{"name":"6Nantong University, Nantong, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Wu","sequence":"additional","affiliation":[{"name":"1Cardiovascular and Metabolic Research Unit, Laurentian University, Ontario, Canada"},{"name":"2School of Human Kinetics, Faculty of Health, Laurentian University, Ontario, Canada"},{"name":"4Health Sciences North Research Institute, Sudbury, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"1Cardiovascular and Metabolic Research Unit, Laurentian University, Ontario, Canada"},{"name":"3Department of Biology, Laurentian University, Ontario, Canada"},{"name":"7Department of Biology, York University, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/248\/1\/JOE-20-0190.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/248\/1\/JOE-20-0190.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:57:14Z","timestamp":1784555834000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/248\/1\/17-30\/5396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/joe-20-0190","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]}}}