{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:32:33Z","timestamp":1784867553212,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,5]]},"abstract":"\n Vitamin D deficiency is associated with symptoms of skeletal muscle myopathy including muscle weakness and fatigue. Recently, vitamin D-related metabolites have been linked to the maintenance of mitochondrial function within skeletal muscle. However, current evidence is limited to\n in vitro<\/jats:italic>\n models and the effects of diet-induced vitamin D deficiency upon skeletal muscle mitochondrial function\n in vivo<\/jats:italic>\n have received little attention. In order to examine the role of vitamin D in the maintenance of mitochondrial function\n in vivo<\/jats:italic>\n , we utilised an established model of diet-induced vitamin D deficiency in C57BL\/6J mice. Mice were either fed a control diet (2200 IU\/kg i.e. vitamin D replete) or a vitamin D-deplete (0 IU\/kg) diet for periods of 1, 2 and 3 months. Gastrocnemius muscle mitochondrial function and ADP sensitivity were assessed via high-resolution respirometry and mitochondrial protein content via immunoblotting. As a result of 3 months of diet-induced vitamin D deficiency, respiration supported via complex I\u2009+\u2009II (CI\u2009+\u2009II\n P<\/jats:sub>\n ) and the electron transport chain (ETC) were 35 and 37% lower when compared to vitamin D-replete mice (\n P<\/jats:italic>\n < 0.05). Despite functional alterations, citrate synthase activity, AMPK phosphorylation, mitofilin, OPA1 and ETC subunit protein content remained unchanged in response to dietary intervention (\n P<\/jats:italic>\n > 0.05). In conclusion, we report that 3 months of diet-induced vitamin D deficiency reduced skeletal muscle mitochondrial respiration in C57BL\/6J mice. Our data, when combined with previous\n in vitro<\/jats:italic>\n observations, suggest that vitamin D-mediated regulation of mitochondrial function may underlie the exacerbated muscle fatigue and performance deficits observed during vitamin D deficiency.\n <\/jats:p>","DOI":"10.1530\/joe-20-0233","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T04:03:20Z","timestamp":1618545800000},"page":"113-124","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":30,"title":["Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration"],"prefix":"10.1530","volume":"249","author":[{"given":"Stephen P","family":"Ashcroft","sequence":"first","affiliation":[{"name":"1School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK"},{"name":"2MRC-Versus Arthritis Centre for Musculoskeletal Ageing Research, Clinical, Metabolic and Molecular Physiology, University of Nottingham, Royal Derby Hospital Centre, Derby, UK"},{"name":"3Mitochondrial Metabolism and Ageing Laboratory, Garvan Institute of Medical Research, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth","family":"Fletcher","sequence":"additional","affiliation":[{"name":"3Mitochondrial Metabolism and Ageing Laboratory, Garvan Institute of Medical Research, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashleigh M","family":"Philp","sequence":"additional","affiliation":[{"name":"3Mitochondrial Metabolism and Ageing Laboratory, Garvan Institute of Medical Research, Sydney, New South Wales, Australia"},{"name":"4St Vincent\u2019s Clinical School, UNSW Medicine, UNSW Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Jenkinson","sequence":"additional","affiliation":[{"name":"5Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK"},{"name":"6ANZAC Research Institute, University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shatarupa","family":"Das","sequence":"additional","affiliation":[{"name":"7Centenary Institute, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia"},{"name":"8UTS Centenary Centre for Inflammation, University Technology Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip M","family":"Hansbro","sequence":"additional","affiliation":[{"name":"7Centenary Institute, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia"},{"name":"8UTS Centenary Centre for Inflammation, University Technology Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J","family":"Atherton","sequence":"additional","affiliation":[{"name":"2MRC-Versus Arthritis Centre for Musculoskeletal Ageing Research, Clinical, Metabolic and Molecular Physiology, University of Nottingham, Royal Derby Hospital Centre, Derby, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Philp","sequence":"additional","affiliation":[{"name":"3Mitochondrial Metabolism and Ageing Laboratory, Garvan Institute of Medical Research, Sydney, New South Wales, Australia"},{"name":"4St Vincent\u2019s Clinical School, UNSW Medicine, UNSW Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/249\/2\/JOE-20-0233.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/249\/2\/JOE-20-0233.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:57:15Z","timestamp":1784555835000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/249\/2\/113-124\/5410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/joe-20-0233","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.15.098087","asserted-by":"object"}]},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5]]}}}