{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:38:30Z","timestamp":1788485910971,"version":"build-2803163510"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:p>\n                    The epithelial cells of choroid plexus (CP) in brain ventricles produce cerebrospinal fluid and act as the blood-cerebrospinal fluid barrier. In this study, we confirmed that CP in the 4th ventricle is composed of cellular oscillators that all harbor glucocorticoid receptors and are mutually synchronized to produce a robust clock gene expression rhythm detectable at the tissue level\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Animals lacking glucocorticoids (GCs) due to surgical removal of adrenal glands had\n                    <jats:italic>Per1<\/jats:italic>\n                    ,\n                    <jats:italic>Per2<\/jats:italic>\n                    ,\n                    <jats:italic>Nr1d1<\/jats:italic>\n                    and\n                    <jats:italic>Bmal1<\/jats:italic>\n                    clock gene rhythmicity in their CP significantly dampened, whereas subjecting them to daily bouts of synthetic GC analog, dexamethasone (DEX), reinforced those rhythms. We verified these\n                    <jats:italic>in vivo<\/jats:italic>\n                    effects using an\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of organotypic CP explants; depending on the time of its application, DEX significantly increased the amplitude and efficiently reset the phase of the CP clock. The results are the first description of a PRC for a non-neuronal clock in the brain, demonstrating that CP clock shares some properties with the non-neuronal clocks elsewhere in the body. Finally, we found that DEX exhibited multiple synergic effects on the CP clock, including acute activation of\n                    <jats:italic>Per1<\/jats:italic>\n                    expression and change of PER2 protein turnover rate. The DEX-induced shifts of the CP clock were partially mediated via PKA-ERK1\/2 pathway. The results provide the first evidence that the GC rhythm strengthens and entrains the clock in the CP helping thus fine-tune the brain environment according to time of day.\n                  <\/jats:p>","DOI":"10.1530\/joe-20-0526","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T10:26:56Z","timestamp":1608632816000},"page":"155-166","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":20,"title":["Glucocorticoids reset circadian clock in choroid plexus via period genes"],"prefix":"10.1530","volume":"248","author":[{"given":"Karol\u00edna","family":"Li\u0161ka","sequence":"first","affiliation":[{"name":"1Laboratory of Biological Rhythms, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic"},{"name":"2Third Faculty of Medicine, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Sl\u00e1dek","sequence":"additional","affiliation":[{"name":"1Laboratory of Biological Rhythms, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vendula","family":"\u010ce\u010dmanov\u00e1","sequence":"additional","affiliation":[{"name":"1Laboratory of Biological Rhythms, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Sumov\u00e1","sequence":"additional","affiliation":[{"name":"1Laboratory of Biological Rhythms, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/248\/2\/JOE-20-0526.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/248\/2\/JOE-20-0526.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:57:24Z","timestamp":1784555844000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/248\/2\/155-166\/5454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/joe-20-0526","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2]]}}}