{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:42:45Z","timestamp":1790242965445,"version":"4.1.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,4]]},"abstract":"\n Vaspin is a novel adipokine mainly expressed in visceral adipose tissue and closely related to obesity and insulin-resistance. Currently, data about its ovarian expression are limited to animal models and its role in human reproduction is largely unexplored. Our study\u2019s aims were then to characterise vaspin expression in the human ovary and to study\n in vitro<\/jats:italic>\n its effects on granulosa cells physiology. Secondly, we assessed vaspin and its receptor GRP78 variations in granulosa cells and follicular fluid of a cohort of 112 infertile women undergoing an\n in vitro<\/jats:italic>\n fertilisation procedure and allocated to three groups, each including normal-weight and obese subjects: 34 PCOS patients, 33 women with isolated polycystic ovary morphology (ECHO group) and 45 controls. Vaspin and GRP78 expression in the ovary was assessed by immunohistochemistry, RT-qPCR and Western blot. Granulosa cells and follicular fluid were analysed by RT-qPCR and ELISA, respectively.\n In vitro<\/jats:italic>\n , granulosa cells metabolism was studied after stimulation with recombinant human vaspin, with and without a siRNA directed against GRP78. Vaspin was highly expressed in the human ovary and concentration-dependently enhanced granulosa cells steroidogenesis, proliferation and viability through GRP78 (\n P<\/jats:italic>\n < 0.0001). Vaspin levels in both granulosa cells and follicular fluid were significantly higher in obese women (\n P<\/jats:italic>\n < 0.0001) and in the normal-weight ECHO group (\n P<\/jats:italic>\n < 0.001), which also had the highest expression rates of GRP78 (\n P<\/jats:italic>\n < 0.05). Although further investigation is needed, vaspin appears as a novel modulator of human granulosa cells physiology and possibly plays a role in PCOS pathogenesis, notably protecting from insulin-resistance induced complications.\n <\/jats:p>","DOI":"10.1530\/joe-20-0550","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T09:42:26Z","timestamp":1613727746000},"page":"57-70","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":23,"title":["Vaspin, a novel adipokine in woman granulosa cells physiology and PCOS pathogenesis?"],"prefix":"10.1530","volume":"249","author":[{"given":"Alice","family":"Bongrani","sequence":"first","affiliation":[{"name":"1UMR 85 Physiology of Reproduction and Behaviour, National Research Institute for Agriculture, Food and Environment (INRAE) Centre Val de Loire, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namya","family":"Mellouk","sequence":"additional","affiliation":[{"name":"1UMR 85 Physiology of Reproduction and Behaviour, National Research Institute for Agriculture, Food and Environment (INRAE) Centre Val de Loire, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Ram\u00e9","sequence":"additional","affiliation":[{"name":"1UMR 85 Physiology of Reproduction and Behaviour, National Research Institute for Agriculture, Food and Environment (INRAE) Centre Val de Loire, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Cornuau","sequence":"additional","affiliation":[{"name":"2Department of Reproductive Medicine and Biology, University Hospital of Tours, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Guerif","sequence":"additional","affiliation":[{"name":"1UMR 85 Physiology of Reproduction and Behaviour, National Research Institute for Agriculture, Food and Environment (INRAE) Centre Val de Loire, Nouzilly, France"},{"name":"2Department of Reproductive Medicine and Biology, University Hospital of Tours, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Froment","sequence":"additional","affiliation":[{"name":"1UMR 85 Physiology of Reproduction and Behaviour, National Research Institute for Agriculture, Food and Environment (INRAE) Centre Val de Loire, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00eblle","family":"Dupont","sequence":"additional","affiliation":[{"name":"1UMR 85 Physiology of Reproduction and Behaviour, National Research Institute for Agriculture, Food and Environment (INRAE) Centre Val de Loire, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/249\/1\/JOE-20-0550.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/249\/1\/JOE-20-0550.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:57:25Z","timestamp":1784555845000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/249\/1\/57-70\/5423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/joe-20-0550","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4]]}}}