{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:21:13Z","timestamp":1784557273908,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Bone healing and regeneration is a complex process that recapitulates embryonic skeletal development and is delayed in diseases like osteoporosis. Bone healing therapies like recombinant bone morphogenetic-2 protein (rhBMP-2) and parathyroid hormone (PTH), an approved bone anabolic therapy reduces fracture risks but are fraught with high cost and several side effects. Thus, there is an unmet need for cost-effective bone healing agents. In this study, we have synthesized 3-piperidinylethoxypterocarpan (3-PEP) which is a hybrid of bone supplement ipriflavone and anti-resorptive drug raloxifene and evaluated its bone regeneration and healing potential. Prior to studies in animal models, the potency of 3-PEP was confirmed in calvarial osteoblast cells. Bromodeoxy uridine cell proliferation and cell viability assay revealed that 3-PEP at 100 pM concentration increased the proliferation and survival of osteoblasts simultaneously inhibiting the apoptosis by involving activation of BCL-2 by phosphorylation at Ser70 site through MEK-ERK pathway.\n                      <jats:italic>In vivo<\/jats:italic>\n                      studies were conducted in estrogen-deficient ovariectomized Balb\/c mice and drill hole injury was generated in the mid diaphysis of the femur in all the animals. Treatment with 3-PEP commenced the next day onward and terminated at 7 and 15 days. Micro-CT analysis and calcein labeling of newly generated bone at the drill hole injury site showed that 3-PEP promotes bone healing and new bone formation at a dose of 5 mg\/kg at the injury site. These data were also corroborated in non-ovariectomized Balb\/c mice cortical defect model. Owing to the side effects associated with rhBMP-2 and PTH, along with the expenses involved, our study proposes an alternative therapeutic option for bone healing.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/joe-21-0439","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T05:05:29Z","timestamp":1653368729000},"page":"51-64","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":3,"title":["3-PEP promotes bone regeneration by up regulating BCL-2 expression via ERK phosphorylation"],"prefix":"10.1530","volume":"254","author":[{"given":"Alok","family":"Tripathi","sequence":"first","affiliation":[{"name":"1Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, 226031, India"},{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallavi","family":"Awasthi","sequence":"additional","affiliation":[{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"},{"name":"3Division of Medicinal & Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, 226031, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kundan Singh","family":"Rawat","sequence":"additional","affiliation":[{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"},{"name":"3Division of Medicinal & Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, 226031, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atma P","family":"Dwivedi","sequence":"additional","affiliation":[{"name":"3Division of Medicinal & Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, 226031, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna Bhan","family":"Singh","sequence":"additional","affiliation":[{"name":"1Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, 226031, India"},{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kriti","family":"Sharma","sequence":"additional","affiliation":[{"name":"1Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, 226031, India"},{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Prakash","sequence":"additional","affiliation":[{"name":"1Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, 226031, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2487-4082","authenticated-orcid":true,"given":"Divya","family":"Singh","sequence":"additional","affiliation":[{"name":"1Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, 226031, India"},{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2758-2461","authenticated-orcid":true,"given":"Atul","family":"Goel","sequence":"additional","affiliation":[{"name":"2Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India"},{"name":"3Division of Medicinal & Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, 226031, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/254\/1\/JOE-21-0439.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/254\/1\/JOE-21-0439.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:57:39Z","timestamp":1784555859000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/254\/1\/51-64\/5513"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/joe-21-0439","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,1]]}}}