{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:54:44Z","timestamp":1790434484966,"version":"4.1.0"},"reference-count":33,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,9,20]]},"abstract":"<jats:p>\n                    The novel adipokine asprosin, produced by the furin enzymatic cleavage of profibrillin 1 protein (encoded by the\n                    <jats:italic>Fbn1<\/jats:italic>\n                    gene), is implicated in regulating many physiological functions, including reproduction in mammals. In males, asprosin is reported to increase sperm density, sperm motility, and steroid production by interacting with Olfr734, which belongs to the G-protein-coupled receptor family (GPCR). In 2023, our group predicted and characterized asprosin\n                    <jats:italic>in silico<\/jats:italic>\n                    for the first time and demonstrated the robust expression of\n                    <jats:italic>fbn1<\/jats:italic>\n                    , and\n                    <jats:italic>furin<\/jats:italic>\n                    in the gonads of teleost spotted snakehead (ss)\n                    <jats:italic>Channa punctata<\/jats:italic>\n                    . Taking it forward, in the current study, we have investigated the effect of asprosin on the testicular functions of the spotted snakehead. As\n                    <jats:italic>C. punctata<\/jats:italic>\n                    is a seasonal breeder, the reproductive-phase-dependent expression of\n                    <jats:italic>fbn1<\/jats:italic>\n                    in the testis was analyzed, showing significant upregulation during the preparatory and post-spawning phases. Additionally, bacterially overexpressed recombinant asprosin of\n                    <jats:italic>C. punctata<\/jats:italic>\n                    was purified to study the effect of ss asprosin on gametogenesis and steroidogenesis.\n                    <jats:italic>Ex vivo<\/jats:italic>\n                    treatment with recombinant asprosin resulted in significant upregulation of spermatogenic marker genes\n                    <jats:italic>pcna<\/jats:italic>\n                    ,\n                    <jats:italic>aldh1a2<\/jats:italic>\n                    ,\n                    <jats:italic>cyp26a1<\/jats:italic>\n                    , and\n                    <jats:italic>sycp3<\/jats:italic>\n                    . Asprosin also enhanced the gene expression of gonadotropin receptors, as well as sex steroid receptors, in addition to steroidogenic genes\n                    <jats:italic>star<\/jats:italic>\n                    and\n                    <jats:italic>cyp17a1<\/jats:italic>\n                    . Further exploring the downstream signaling cascade, the second messenger of GPCRs, cAMP levels following asprosin treatment were analyzed. Asprosin treatment prominently enhanced cAMP levels, thereby indicating the involvement of GPCR in the transduction of asprosin action. Hence, the study elucidates the regulation of male reproductive function by asprosin in spotted snakeheads.\n                  <\/jats:p>","DOI":"10.1530\/joe-24-0146","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T07:44:00Z","timestamp":1726818240000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":6,"title":["Asprosin regulates male reproduction in teleosts: an in vitro study in Channa punctata"],"prefix":"10.1530","volume":"263","author":[{"given":"Priyanka","family":"Sathoria","sequence":"first","affiliation":[{"name":"Department of Zoology, Maitreyi College, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhawna","family":"Chuphal","sequence":"additional","affiliation":[{"name":"Department of Zoology, Miranda House, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2684-4311","authenticated-orcid":true,"given":"Umesh","family":"Rai","sequence":"additional","affiliation":[{"name":"University of Jammu, Jammu, Jammu and Kashmir, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9140-4032","authenticated-orcid":true,"given":"Brototi","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Zoology, Maitreyi College, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Fish Physiology and Biochemistry","first-page":"1249","article-title":"Seasonality of reproduction in male spotted murrel Channa punctatus: correlation of environmental variables and plasma sex steroids with histological changes in testis","volume":"42","author":"Basak","year":"2016","unstructured":"Basak RRoy A & Rai U 2016 Seasonality of reproduction in male spotted murrel Channa punctatus: correlation of environmental variables and plasma sex steroids with histological changes in testis. 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