{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:41:51Z","timestamp":1767915711325,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1979,5,1]],"date-time":"1979-05-01T00:00:00Z","timestamp":294364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,5,1]]},"abstract":"<jats:p>Summary. The cyclic AMP\u2013phosphodiesterase (EC 3.1.4.17) of buffalo spermatozoa is distributed in the head, mid-piece and tail fractions and has multiple forms, 70% of which is in the bound form. The bound enzyme was not solubilized by Triton X-100, lubrol or hyamine 2389. Kinetic measurements of the soluble enzyme showed two apparent Km values for low and high cAMP concentrations, i.e. 4\u00b75 and 100 \u03bcm with Vmax values of 0\u00b725 and 2\u00b70 nmol cAMP hydrolysed min\u22121 mg protein\u22121. The bound enzyme had an apparent Km of 66\u00b76 \u03bcm with a Vmax of 0\u00b775 nmol cAMP hydrolysed min\u22121 mg protein\u22121. The pH for optimum enzyme activity was 7\u00b75 and Mg2+ was essential for the activity of the soluble and bound enzymes. Methylxanthines, ATP, ADP and PPi inhibited the soluble and bound enzymes, ATP being the most potent inhibitor.<\/jats:p>","DOI":"10.1530\/jrf.0.0560133","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T03:12:46Z","timestamp":1079752366000},"page":"133-139","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":16,"title":["Adenosine 3\u20325\u2032-monophosphate phosphodiesterase of buffalo spermatozoa"],"prefix":"10.1093","volume":"56","author":[{"given":"S. K.","family":"Bhatnagar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. S.","family":"Chaudhry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Anand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1979,5,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/56\/1\/133\/64933041\/jrf_56_1_021.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/56\/1\/133\/64933041\/jrf_56_1_021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:16:32Z","timestamp":1767896192000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/56\/1\/133\/8290010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,5,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,5,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.0560133","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1979,5,1]]},"published":{"date-parts":[[1979,5,1]]}}}