{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:45:40Z","timestamp":1767915940738,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1980,7,1]],"date-time":"1980-07-01T00:00:00Z","timestamp":331257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,7,1]]},"abstract":"<jats:p>Summary. Immunological and biochemical methods were employed to demonstrate the presence of progestagen-dependent proteins in human endometrium. Cytosols were prepared from proliferative and secretory phase endometria of cycling women, from decidua and decidua-rich tissues of women in early pregnancy and from decidua of tubal pregnancy. Antisera were raised in rabbits against the antigens of decidua of tubal pregnancy and decidua-rich tissues. Immunoelectrophoresis, Ouchterlony's immunodiffusion test and polyacrylamide gel electrophoresis using native gels revealed 2 antigenic proteins, designated antigens A and B, in secretory endometria, decidua-rich tissues, decidua, and in decidua of tubal pregnancy. However, only 1 antigenic protein was detected by SDS-gel electrophoresis: antigens A and B may therefore be two different proteins or two forms of a single protein. The antigens could not be detected in non-pregnancy sera or in term placentae. Double isotopic labelling (incubation of tissues with [3H]- and [14C]leucine) followed by protein fractionation methods were used to compare the in-vitro synthesis rates of antigens in proliferative tissues with those in decidua or secretory endometria. The rate of synthesis of antigens A and B was markedly higher in the decidua and secretory endometria than in the proliferative endometria. We conclude, therefore, that during progestagen-dependent transformation of proliferative phase endometria into secretory endometria and decidua in women, there is a selective stimulation of at least one species of pregnancy-associated protein.<\/jats:p>","DOI":"10.1530\/jrf.0.0590273","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"273-285","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":56,"title":["Detection and synthesis of a progestagen-dependent protein in human endometrium"],"prefix":"10.1093","volume":"59","author":[{"given":"S. G.","family":"Joshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. M.","family":"Ebert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Swartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1980,7,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/59\/2\/273\/64934768\/jrf_59_2_004.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/59\/2\/273\/64934768\/jrf_59_2_004.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:17:35Z","timestamp":1767896255000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/59\/2\/273\/8290235"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,7,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,7,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.0590273","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1980,7,1]]},"published":{"date-parts":[[1980,7,1]]}}}