{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:02:18Z","timestamp":1767855738630,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1985,11,1]],"date-time":"1985-11-01T00:00:00Z","timestamp":499651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,11,1]]},"abstract":"<jats:title>Summary.<\/jats:title>\n                  <jats:p>The platelet-activating factor (PAF) produced by mouse embryos showed similar kinetics of action and dose\u2014response curve, in a bioassay, as did 1-0-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine (PAF-acether). The activity of the embryo-derived PAF was not affected by inhibitors of the ADP (pyruvate kinase with phosphoenol pyruvate) or cyclo-oxygenase (indomethacin) pathways of platelet activation. Chlorpromazine, an inhibitor of the PAF-acether pathway of platelet activation, caused a significant inhibition of the effects of embryo-derived PAF. Phospholipases A2, C and D significantly inhibited the activity while lipase had no effect, suggesting a phospholipid structure. All the embryo-derived PAF was found in the chloroform fraction after chloroform:methanol (2:1 v\/v) extraction, as was PAF-acether. Both factors migrated at a similar rate (Rf0\u00b710\u20130\u00b712) on silica thin-layer chromatography (chloroform:methanol:water; 65:35:4 by vol.). The embryo-derived PAF therefore displays chemical, biochemical and physiological properties similar to those of PAF-acether.<\/jats:p>","DOI":"10.1530\/jrf.0.0750375","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"375-380","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":110,"title":["Partial characterization of the embryo-derived platelet-activating factor in mice"],"prefix":"10.1093","volume":"75","author":[{"given":"C.","family":"O'Neill","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1985,11,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/75\/2\/375\/64852287\/jrf_75_2_006.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/75\/2\/375\/64852287\/jrf_75_2_006.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T21:14:35Z","timestamp":1767820475000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/75\/2\/375\/8282991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,11,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,11,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.0750375","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1985,11,1]]},"published":{"date-parts":[[1985,11,1]]}}}