{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:21:53Z","timestamp":1787170913064,"version":"build-2736575974"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1987,9,1]],"date-time":"1987-09-01T00:00:00Z","timestamp":557452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,9,1]]},"abstract":"<jats:p>Summary. In Exp. 1, maternal (caruncle) and fetal (cotyledon) portions of the placenta as well as uterine endometrium were obtained from cows at mid-gestation and evaluated for angiogenic activity by placing tissue samples on chick chorioallantoic membranes (CAM). Only caruncular tissues exhibited angiogenic activity in the CAM assay. In Exp. 2, lyophilized homogenates of caruncular tissues obtained from cows at mid-gestation were evaluated for angiogenic activity on CAM and for their ability to stimulate mitosis of bovine aortic endothelial cells in vitro. Homogenates of caruncular tissues again were angiogenic on the CAM and also were mitogenic for endothelial cells. In Exp. 3, maternal (caruncle and endometrium) and fetal (cotyledon and fetal membrane) portions of the placenta were obtained from cows at mid-gestation and fine minces (explants) of each were cultured for 24 h. Explant-conditioned media were then tested for angiogenic activity by their abilities to stimulate mitosis and migration of bovine aortic endothelial cells in vitro. Conditioned media from caruncular explants, but not from explants of other tissues, exhibited both mitogenic and migration-stimulating activities. When pools of caruncular explant-conditioned media were fractionated by ultrafiltration, mitogenic activity was not present in fractions of Mr &amp;lt; 10 000, &amp;lt; 30 000 and &amp;lt; 100 000, but was retained in fractions of Mr &amp;gt; 10 000, &amp;gt; 30 000 and &amp;gt; 100 000. Mitogenic activity was not observed in any fractions subjected to heat treatment. On the basis of these data, we conclude that, during mid-gestation, angiogenic activity of bovine placenta is associated primarily with the maternal caruncular portion of the placenta which, therefore, may direct growth of the placental microvasculature. This angiogenic activity seems to have a molecular weight of &amp;gt; 100 000 and be heat labile.<\/jats:p>","DOI":"10.1530\/jrf.0.0810233","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"233-240","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Angiogenic activity of placental tissues of cows"],"prefix":"10.1093","volume":"81","author":[{"given":"L. P.","family":"Reynolds","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. S.","family":"Millaway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. D.","family":"Kirsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Infeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"Redmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1987,9,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/81\/1\/233\/64812447\/jrf_81_1_027.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/81\/1\/233\/64812447\/jrf_81_1_027.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:17:43Z","timestamp":1767813463000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/81\/1\/233\/8278873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,9,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.0810233","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1987,9,1]]},"published":{"date-parts":[[1987,9,1]]}}}