{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T20:58:04Z","timestamp":1767819484630,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1990,7,1]],"date-time":"1990-07-01T00:00:00Z","timestamp":646790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,7,1]]},"abstract":"<jats:p>Summary. Fluid was aspirated from the preovulatory follicles of mares before and 12, 24 and 36 h after intravenous administration of hCG. Follicular fluid significantly (P &amp;lt; 0\u00b7001) reduced lymphocyte blastogenesis in vitro and, at a dilution of 1:100, fluid collected at 36 h after administration of hCG was significantly more suppressive (P &amp;lt; 0\u00b701) than fluid collected before 36 h. Suppression of blastogenesis was reduced by extracting the follicular fluid with ether or by charcoal treatment (P &amp;lt; 0\u00b701) or by heating at 56\u00b0C for 30 min (P &amp;lt; 0\u00b705). Preincubation of lymphocytes with 2 of 5 follicular fluid samples expressed subsequent blastogenesis. Follicular fluid inhibited blastogenesis of T-cell growth factor (TCGF)-dependent Con A lymphoblasts (P &amp;lt; 0\u00b705) and the degree of inhibition was related to time of addition of the TCGF and time of collection of the follicular fluid. These results indicate that preovulatory follicular fluid in the mare is increasingly suppressive to lymphocytes as time of ovulation approaches and that this immunosuppression is associated with an alteration of the response to lymphokine stimulation.<\/jats:p>\n                  <jats:p>Keywords: mare; immunosuppression; follicular fluid<\/jats:p>","DOI":"10.1530\/jrf.0.0890627","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"627-632","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Immunosuppressive properties of follicular fluid from preovulatory horse follicles"],"prefix":"10.1093","volume":"89","author":[{"given":"E. D.","family":"Watson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. G.","family":"Zanecosky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1990,7,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/89\/2\/627\/64816787\/jrf_89_2_030.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/89\/2\/627\/64816787\/jrf_89_2_030.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T20:05:24Z","timestamp":1767816324000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/89\/2\/627\/8279504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,7,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,7,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.0890627","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1990,7,1]]},"published":{"date-parts":[[1990,7,1]]}}}