{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:13:23Z","timestamp":1767809603918,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1991,11,1]],"date-time":"1991-11-01T00:00:00Z","timestamp":688953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,11,1]]},"abstract":"<jats:p>Summary. Melittin, an activator of phospholipase (PL) A-2, increased the outputs of prostaglandin (PG) F-2\u03b1 and 6-keto-PGF-1\u03b1, but not of PGE-2, from Day-7 guineapig uterus superfused in vitro. Reducing the extracellular calcium concentration (by omitting calcium chloride from the superfusing fluid) partially inhibited the stimulatory effect of melittin on uterine PG production. TMB-8 (an intracellular calcium antagonist) completely prevented the stimulation of PGF-2\u03b1 and 6-keto-PGF-1\u03b1 output by melittin, although the production of both PGs tended to increase after stopping the melittin and TMB-8 treatments. TMB-8 also inhibited the increases in outputs of PGF-2\u03b1, 6-keto-PGF-1\u03b1 and PGE-2 and prevented contraction of the uterus induced by exogenous PLA-2. Trifluoperazine (a calmodulin antagonist) had no inhibitory effect on the increases in outputs of PGF-2\u03b1 and 6-keto-PGF-1\u03b1 produced by melittin; it potentiated the stimulatory effect of melittin on 6-keto-PGF-1\u03b1 output and allowed melittin to increase PGE-2 output. When melittin was applied twice to the superfused uterus with an interval of 1 h between each treatment, partial refractoriness of the responses to melittin was seen: the magnitudes of the increases in PGF-2\u03b1 and 6-keto-PGF-1\u03b1 outputs were 40\u201350% less after the second treatment than after the first treatment. These results show that melittin stimulates the synthesis of PGF-2\u03b1 and PGI-2 (measured as 6-keto-PGF-1\u03b1) in guinea-pig uterus by mechanisms which are calcium dependent. The results are compatible with there being a protein of similar functional activity to melittin in the guinea-pig uterus, which may be involved in the stimulation of endometrial PGF-2\u03b1 synthesis.<\/jats:p>\n                  <jats:p>Keywords: melittin; TMB-8; trifluoperazine; prostaglandins; guinea-pig; uterus<\/jats:p>","DOI":"10.1530\/jrf.0.0930577","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"577-583","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of melittin on prostaglandin production by guinea-pig uterus"],"prefix":"10.1093","volume":"93","author":[{"given":"F. A.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. L.","family":"Poyser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1991,11,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/93\/2\/577\/64565541\/jrf_93_2_039.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/93\/2\/577\/64565541\/jrf_93_2_039.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:04:10Z","timestamp":1767805450000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/93\/2\/577\/8211565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,11,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,11,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.0930577","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1991,11,1]]},"published":{"date-parts":[[1991,11,1]]}}}