{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T17:47:31Z","timestamp":1781804851875,"version":"3.54.5"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1994,5,1]],"date-time":"1994-05-01T00:00:00Z","timestamp":767750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ovarian response to equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG), the effect of timing of anaesthesia relative to hCG injection and the use of laparoscopic intrauterine artificial insemination were examined in the puma (Fells concolor). In Expt 1, females were treated with 100 (n = 6) or 200 (n = 8) iu eCG (i.m.) followed 80 h later by 100 iu hCG (i.m.) and were then anaesthetized 40\u201343 h after hCG injection for ovarian assessment. Although there was no difference (P &amp;gt; 0.05) in the number of unovulated ovarian follicles, females treated with 200 iu eCG had more (P &amp;lt; 0.05) corpora lutea per female and more corpora lutea as a percentage of the total number of ovarian structures. In Expt 2, all females were treated with 200 iu eCG and 80 h later with 100 iu hCG, and then anaesthetized either 31\u201339 h (Group A; n = 8) or 41\u201350 h (Group B; n = 6) after hCG injection for ovarian assessment. All Group B pumas ovulated compared with only three (37.5%) Group A females (P&amp;lt; 0.05). Compared with Group A, Group B pumas had more corpora lutea per female, more corpora lutea as a percentage of the total number of ovarian structures, and fewer unovulated follicles (P &amp;lt; 0.05). One of nine post-ovulatory females laparoscopically inseminated in utero with 16 \u00d7 106 motile spermatozoa became pregnant and delivered a healthy cub. Administration of 200 iu eCG and 100 iu hCG followed by anaesthesia no earlier than 41 h after hCG treatment is most likely to result in ovulation in pumas, and laparoscopic artificial insemination can be used to produce pregnancy in this species.<\/jats:p>","DOI":"10.1530\/jrf.0.1010103","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"103-108","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":47,"title":["Gonadotrophin dose and timing of anaesthesia for laparoscopic artificial insemination in the puma (\n                    <i>Felis concolor<\/i>\n                    )"],"prefix":"10.1093","volume":"101","author":[{"given":"M A","family":"Barone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D E","family":"Wildt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A P","family":"Byers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M E","family":"Roelke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C M","family":"Glass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J G","family":"Howard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1994,5,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/101\/1\/103\/64785071\/jrf_101_1_015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/101\/1\/103\/64785071\/jrf_101_1_015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:22:09Z","timestamp":1767810129000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/101\/1\/103\/8264292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,5,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.1010103","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1994,5,1]]},"published":{"date-parts":[[1994,5,1]]}}}