{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:48:29Z","timestamp":1767815309037,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1994,9,1]],"date-time":"1994-09-01T00:00:00Z","timestamp":778377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A patch-clamp study of Ca2+ currents and spectrofluorometric detection of the intracellular Ca2+ concentration [Ca2+]i was performed on porcine myometrial cells that had been isolated by collagenase. Isolated myometrial cells were the typical long cylinder shape. The main length and diameter of myometrial cells were 505 \u00b1 20 and 11\u00b1 0.5 \u03bcm (n = 40), respectively, in the prepartum period and 265 \u00b1 22 and 7 \u00b1 0.4 \u03bcm (n = 40), respectively, in the luteal phase. Immunocytochemistry with an antibody against desmin stained 90% of the cells positively, and about 95% of the cells excluded Trypan blue dye. The basal [Ca2+]i of myometrial cells in the luteal phase and the prepartum period was 119 \u00b1 12 (n = 30) and 154 \u00b1 31 nmol l\u22121 (n = 48), respectively. In prepartum myometrial cells, oxytocin (10\u22127 mol l\u22121) and carbachol (10\u22124 mol l\u22121) increased [Ca2+]i in a biphasic pattern, with a sharp peak followed by a plateau. In cells in the luteal phase, adrenaline (10\u22127 mol l\u22121) plus propranolol (10\u22126 mol l\u22121) produced a biphasic increase in [Ca2+]i. However, in the absence of propranolol, the increase in [Ca2+]i by adrenaline was small. Prostaglandin F2\u03b1 (10\u22126 mol l\u22121) induced a monophasic increase in the [Ca2+]i in cells in the luteal phase. By depolarizing the cells from \u221230 to +50 mV from a holding potential of \u221250 mV, Ca2+ currents were evoked with a threshold at \u221220 mV, reaching a maximum between 10 and 30 mV. BayK8644 (10\u22127 mol l\u22121), an L-type Ca2+ channel agonist, and oxytocin (10\u22127 mol l\u22121) enhanced Ca2+ currents by 166 \u00b1 64% and 41 \u00b1 10%, respectively, in prepartum cells. These results suggest that freshly dispersed porcine myometrial cells contain an intact membrane and possess functional voltage-dependent Ca2+ channels and receptors for major physiological regulators. Thus, porcine myometrial cells provide a useful model for the study of excitation\u2013contraction coupling and the influence of physiological regulators in the myometrium.<\/jats:p>","DOI":"10.1530\/jrf.0.1020049","type":"journal-article","created":{"date-parts":[[2004,3,20]],"date-time":"2004-03-20T01:55:52Z","timestamp":1079747752000},"page":"49-55","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of freshly dispersed porcine myometrial cells: evidence for voltage-dependent Ca2+ channels and regulatory receptors"],"prefix":"10.1093","volume":"102","author":[{"given":"R","family":"ZhuGe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W H","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1994,9,1]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/102\/1\/49\/64785489\/jrf_102_1_007.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/102\/1\/49\/64785489\/jrf_102_1_007.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:30:55Z","timestamp":1767810655000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/102\/1\/49\/8264568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,9,1]]}},"URL":"https:\/\/doi.org\/10.1530\/jrf.0.1020049","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1994,9,1]]},"published":{"date-parts":[[1994,9,1]]}}}