{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:24:57Z","timestamp":1789154697448,"version":"build-2803163510"},"reference-count":38,"publisher":"Bioscientifica","issue":"2","license":[{"start":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T00:00:00Z","timestamp":1618963200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["raf.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,4,21]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine if models of human 'receptive' and 'non-receptive endometrium' differ in their responses to nitric oxide (NO) supplementation by measuring the levels of the enzymes of the endocannabinoid system (ECS) (fatty acid amide hydrolase (FAAH) and N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD)), which control the 'anandamide tone' essential for successful pregnancy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>\n                      A study of FAAH and NAPE-PLD expression (using human endometrium) through the menstrual cycle and an\n                      <jats:italic>in vitro<\/jats:italic>\n                      using a model of 'receptive' (Ishikawa) and 'non-receptive' (HEC-1A) human endometrial cell lines treated with the NO-donating compound S-nitroso-N-acetylpenicillamine (SNAP).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Immunoreactivity measured by optimised H-score for both FAAH and NAPE-PLD was reduced in secretory (receptive) endometrium compared to proliferative (non-receptive) endometrium (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009= 0.0009 and &lt;0.0001, respectively).\n                      <jats:italic>FAAH<\/jats:italic>\n                      and\n                      <jats:italic>NAPE<\/jats:italic>\n                      transcript levels were significantly higher in untreated Ishikawa cells than in HEC-1A cells (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009= 0.0228 and 0.0001, respectively). Treatment of cultures with SNAP resulted in an increase in the amount of\n                      <jats:italic>FAAH<\/jats:italic>\n                      mRNA produced by Ishikawa cells and a decrease in\n                      <jats:italic>NAPE-PLD<\/jats:italic>\n                      mRNA. No effect of SNAP was observed in HEC-1A cells. Similarly, FAAH protein was significantly decreased in endometria representative of the receptive endometrium.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These data suggest that NO most likely affects the expression of ECS enzymes in the implantation site of a receptive endometrium; a phenomenon not seen in a non-receptive endometrium. These effects are most marked with FAAH expression, suggesting that FAAH may play the more critical role in ensuring the correct 'anandamide tone' for successful embryo implantation than NAPE-PLD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Lay summary<\/jats:title>\n                    <jats:p>Embryo implantation into the wall of the uterus is only successful when the inner wall of the uterus (the endometrium) is \u2018receptive\u2019, because if it is \u2018non-receptive\u2019, implantation will fail. Previous work showed that enzymes of the 'endocannabinoid system' are critical for implantation by maintaining the correct level of a fat called anandamide. This is by balancing its synthesis (by N-acylphosphatidylethanolamine specific phospholipase D, NAPE-PLD) and degradation (by fatty acid amide hydrolase, FAAH). Using immortalised cell lines as models of \u2018receptive\u2019 and \u2018non-receptive\u2019 human endometrium, we demonstrate a key stimulator of implantation, nitric oxide, has a positive effect on implantation by both increasing the mRNA levels of the degrading enzyme (FAAH) and decreasing the expression of the synthesising enzyme (NAPE-PLD). These effects are most marked with the degrading enzyme, suggesting that FAAH plays a more critical role than NAPE-PLD in ensuring the correct 'anandamide tone' for successful embryo implantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/raf-20-0035","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T13:34:16Z","timestamp":1617024856000},"page":"107-116","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-13","source":"Crossref","is-referenced-by-count":5,"title":["Nitric oxide positively affects endometrial receptivity via FAAH and NAPE-PLD in vitro"],"prefix":"10.1530","volume":"2","author":[{"given":"Sarah E","family":"Melford","sequence":"first","affiliation":[{"name":"Endocannabinoid Research Group, Reproductive Sciences Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony H","family":"Taylor","sequence":"additional","affiliation":[{"name":"Endocannabinoid Research Group, Reproductive Sciences Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin C","family":"Konje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Frontiers in Physiology","article-title":"Crosstalk between nitric oxide and endocannabinoid signaling pathways in normal and pathological placentation","volume":"9","author":"Aban","year":"2018","unstructured":"Aban CEAccialini PLEtcheverry TLeguizamon GFMartinez NAFarina MG2018 Crosstalk between nitric oxide and endocannabinoid signaling pathways in normal and pathological placentation. Frontiers in Physiology 9 1699. (https:\/\/doi.org\/10.3389\/fphys.2018.01699)"},{"key":"bib2","series-title":"Frontiers in Oncology","article-title":"Expression and function of the endocannabinoid modulating enzymes fatty acid amide hydrolase and N-acylphosphatidylethanolamine-specific phospholipase D in endometrial carcinoma","volume":"9","author":"Ayakannu","year":"2019","unstructured":"Ayakannu TTaylor AHBari MMastrangelo NMaccarrone MKonje JC2019 Expression and function of the endocannabinoid modulating enzymes fatty acid amide hydrolase and N-acylphosphatidylethanolamine-specific phospholipase D in endometrial carcinoma. Frontiers in Oncology 9 1363. (https:\/\/doi.org\/10.3389\/fonc.2019.01363)"},{"key":"bib3","series-title":"Pharmaceuticals","first-page":"2910","article-title":"From fertilisation to implantation in mammalian pregnancy \u2013 modulation of early human reproduction by the endocannabinoid system","volume":"3","author":"Bambang","year":"2010","unstructured":"Bambang KNKarasu TGebeh ATaylor AHMarczylo THLam PWillets JMKonje JC2010 From fertilisation to implantation in mammalian pregnancy \u2013 modulation of early human reproduction by the endocannabinoid system. Pharmaceuticals 3 2910\u20132929. (https:\/\/doi.org\/10.3390\/ph3092910)"},{"key":"bib4","series-title":"Journal of Clinical Endocrinology and Metabolism","first-page":"136","article-title":"Characterization of integrin expression in a well differentiated endometrial adenocarcinoma cell line (Ishikawa)","volume":"82","author":"Castelbaum","year":"1997","unstructured":"Castelbaum AJYing LSomkuti SGSun JIlesanmi AOLessey BA1997 Characterization of integrin expression in a well differentiated endometrial adenocarcinoma cell line (Ishikawa). Journal of Clinical Endocrinology and Metabolism 82 136\u2013142. (https:\/\/doi.org\/10.1210\/jcem.82.1.3658)"},{"key":"bib5","series-title":"Human Reproduction","first-page":"96","article-title":"Role of nitric oxide in implantation and menstruation","volume":"15","author":"Chwalisz","year":"2000","unstructured":"Chwalisz KGarfield RE2000 Role of nitric oxide in implantation and menstruation. Human Reproduction 15 (Supplement 3) 96\u2013111. (https:\/\/doi.org\/10.1093\/humrep\/15.suppl_3.96)"},{"key":"bib6","series-title":"Life Sciences","first-page":"2259","article-title":"Nitric oxide synthesis inhibition suppresses implantation and decreases cGMP concentration and protein peroxidation","volume":"65","author":"Duran-Reyes","year":"1999","unstructured":"Duran-Reyes GGomez-Melendez MRMorali-De La Brena GMercado-Pichardo EMedina-Navarro RHicks-Gomez JJ1999 Nitric oxide synthesis inhibition suppresses implantation and decreases cGMP concentration and protein peroxidation. Life Sciences 65 2259\u20132268. (https:\/\/doi.org\/10.1016\/s0024-3205(99)00491-9)"},{"key":"bib7","series-title":"PLoS ONE","article-title":"Localisation and function of the endocannabinoid system in the human ovary","volume":"4","author":"El-Talatini","year":"2009a","unstructured":"El-Talatini MRTaylor AHElson JCBrown LDavidson ACKonje JC2009a Localisation and function of the endocannabinoid system in the human ovary. PLoS ONE 4 e4579. (https:\/\/doi.org\/10.1371\/journal.pone.0004579)"},{"key":"bib8","series-title":"Human Reproduction","first-page":"1989","article-title":"Fluctuation in anandamide levels from ovulation to early pregnancy in in-vitro fertilization-embryo transfer women, and its hormonal regulation","volume":"24","author":"El-Talatini","year":"2009b","unstructured":"El-Talatini MRTaylor AHKonje JC2009b Fluctuation in anandamide levels from ovulation to early pregnancy in in-vitro fertilization-embryo transfer women, and its hormonal regulation. Human Reproduction 24 1989\u20131998. (https:\/\/doi.org\/10.1093\/humrep\/dep065)"},{"key":"bib9","series-title":"Journal of Physiology and Biochemistry","first-page":"261","article-title":"Cannabinoid-induced cell death in endometrial cancer cells: involvement of TRPV1 receptors in apoptosis","volume":"74","author":"Fonseca","year":"2018","unstructured":"Fonseca BMCorreia-Da-Silva GTeixeira NA2018 Cannabinoid-induced cell death in endometrial cancer cells: involvement of TRPV1 receptors in apoptosis. Journal of Physiology and Biochemistry 74 261\u2013272. (https:\/\/doi.org\/10.1007\/s13105-018-0611-7)"},{"key":"bib10","series-title":"Journal of Clinical Endocrinology and Metabolism","first-page":"2827","article-title":"Ectopic pregnancy is associated with high anandamide levels and aberrant expression of FAAH and CB1 in fallopian tubes","volume":"97","author":"Gebeh","year":"2012","unstructured":"Gebeh AKWillets JMMarczylo ELTaylor AHKonje JC2012 Ectopic pregnancy is associated with high anandamide levels and aberrant expression of FAAH and CB1 in fallopian tubes. Journal of Clinical Endocrinology and Metabolism 97 2827\u20132835. (https:\/\/doi.org\/10.1210\/jc.2012-1780)"},{"key":"bib11","series-title":"Journal of Biological Chemistry","first-page":"23429","article-title":"N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation","volume":"280","author":"Guo","year":"2005","unstructured":"Guo YWang HOkamoto YUeda NKingsley PJMarnett LJSchmid HHDas SKDey SK2005 N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation. Journal of Biological Chemistry 280 23429\u201323432. (https:\/\/doi.org\/10.1074\/jbc.C500168200)"},{"key":"bib12","series-title":"Life Sciences","first-page":"1963","article-title":"Endogenous cannabinoids: metabolism and their role in reproduction","volume":"70","author":"Habayeb","year":"2002","unstructured":"Habayeb OMBell SCKonje JC2002 Endogenous cannabinoids: metabolism and their role in reproduction. Life Sciences 70 1963\u20131977. (https:\/\/doi.org\/10.1016\/s0024-3205(01)01539-9)"},{"key":"bib13","series-title":"Endocrinology","first-page":"5052","article-title":"Expression of the endocannabinoid system in human first trimester placenta and its role in trophoblast proliferation","volume":"149","author":"Habayeb","year":"2008","unstructured":"Habayeb OMTaylor AHBell SCTaylor DJKonje JC2008 Expression of the endocannabinoid system in human first trimester placenta and its role in trophoblast proliferation. Endocrinology 149 5052\u20135060. (https:\/\/doi.org\/10.1210\/en.2007-1799)"},{"key":"bib14","series-title":"Biology of Reproduction","first-page":"235","article-title":"Models for study of human embryo implantation: choice of cell lines?","volume":"82","author":"Hannan","year":"2010","unstructured":"Hannan NJPaiva PDimitriadis ESalamonsen LA2010 Models for study of human embryo implantation: choice of cell lines? Biology of Reproduction 82 235\u2013245. (https:\/\/doi.org\/10.1095\/biolreprod.109.077800)"},{"key":"bib15","series-title":"Journal of Medicinal Chemistry","first-page":"3032","article-title":"Two new unsaturated fatty acid ethanolamides in brain that bind to the cannabinoid receptor","volume":"36","author":"Hanus","year":"1993","unstructured":"Hanus LGopher AAlmog SMechoulam R1993 Two new unsaturated fatty acid ethanolamides in brain that bind to the cannabinoid receptor. Journal of Medicinal Chemistry 36 3032\u20133034. (https:\/\/doi.org\/10.1021\/jm00072a026)"},{"key":"bib16","series-title":"Reproduction","first-page":"733","article-title":"The effects of sex steroid hormones and interleukin-1-beta on MUC1 expression in endometrial epithelial cell lines","volume":"131","author":"Horne","year":"2006","unstructured":"Horne AWLalani ENMargara RAWhite JO2006 The effects of sex steroid hormones and interleukin-1-beta on MUC1 expression in endometrial epithelial cell lines. Reproduction 131 733\u2013742. (https:\/\/doi.org\/10.1530\/rep.1.00883)"},{"key":"bib17","series-title":"Human Reproduction Update","first-page":"347","article-title":"The role of sex steroid hormones, cytokines and the endocannabinoid system in female fertility","volume":"17","author":"Karasu","year":"2011","unstructured":"Karasu TMarczylo THMaccarrone MKonje JC2011 The role of sex steroid hormones, cytokines and the endocannabinoid system in female fertility. Human Reproduction Update 17 347\u2013361. (https:\/\/doi.org\/10.1093\/humupd\/dmq058)"},{"key":"bib18","series-title":"Reviews in Endocrine and Metabolic Disorders","first-page":"145","article-title":"Nitric oxide and its role in blastocyst implantation","volume":"3","author":"Khorram","year":"2002","unstructured":"Khorram O2002 Nitric oxide and its role in blastocyst implantation. Reviews in Endocrine and Metabolic Disorders 3 145\u2013149. (https:\/\/doi.org\/10.1023\/a:1015459029397)"},{"key":"bib19","series-title":"Prostaglandins, Leukotrienes, and Essential Fatty Acids","first-page":"211","article-title":"Oxidative metabolism of endocannabinoids","volume":"66","author":"Kozak","year":"2002","unstructured":"Kozak KRMarnett LJ2002 Oxidative metabolism of endocannabinoids. Prostaglandins, Leukotrienes, and Essential Fatty Acids 66 211\u2013220. (https:\/\/doi.org\/10.1054\/plef.2001.0359)"},{"key":"bib20","series-title":"Journal of Molecular Cell Biology","first-page":"91","article-title":"The endocannabinoid system: \u2018NO\u2019 longer anonymous in the control of nitrergic signalling?","volume":"9","author":"Lipina","year":"2017","unstructured":"Lipina CHundal HS2017 The endocannabinoid system: \u2018NO\u2019 longer anonymous in the control of nitrergic signalling? Journal of Molecular Cell Biology 9 91\u2013103. (https:\/\/doi.org\/10.1093\/jmcb\/mjx008)"},{"key":"bib21","series-title":"Methods","first-page":"402","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","unstructured":"Livak KJSchmittgen TD2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 402\u2013408. (https:\/\/doi.org\/10.1006\/meth.2001.1262)"},{"key":"bib22","series-title":"Progress in Lipid Research","first-page":"344","article-title":"Endocannabinoids: friends and foes of reproduction","volume":"48","author":"Maccarrone","year":"2009","unstructured":"Maccarrone M2009 Endocannabinoids: friends and foes of reproduction. Progress in Lipid Research 48 344\u2013354. (https:\/\/doi.org\/10.1016\/j.plipres.2009.07.001)"},{"key":"bib23","series-title":"Journal of Immunology","first-page":"7183","article-title":"Progesterone up-regulates anandamide hydrolase in human lymphocytes: role of cytokines and implications for fertility","volume":"166","author":"Maccarrone","year":"2001","unstructured":"Maccarrone MValensise HBari MLazzarin NRomanini CFinazzi-Agro A2001 Progesterone up-regulates anandamide hydrolase in human lymphocytes: role of cytokines and implications for fertility. Journal of Immunology 166 7183\u20137189. (https:\/\/doi.org\/10.4049\/jimmunol.166.12.7183)"},{"key":"bib24","series-title":"Human Reproduction Update","first-page":"586","article-title":"The fundamental role of the endocannabinoid system in endometrium and placenta: implications in pathophysiological aspects of uterine and pregnancy disorders","volume":"26","author":"Maia","year":"2020","unstructured":"Maia JFonseca BMTeixeira NCorreia-Da-Silva G2020 The fundamental role of the endocannabinoid system in endometrium and placenta: implications in pathophysiological aspects of uterine and pregnancy disorders. Human Reproduction Update 26 586\u2013602. (https:\/\/doi.org\/10.1093\/humupd\/dmaa005)"},{"key":"bib25","series-title":"Human Reproduction Update","first-page":"415","article-title":"Of mice and (wo)men: factors influencing successful implantation including endocannabinoids","volume":"20","author":"Melford","year":"2014","unstructured":"Melford SETaylor AHKonje JC2014 Of mice and (wo)men: factors influencing successful implantation including endocannabinoids. Human Reproduction Update 20 415\u2013428. (https:\/\/doi.org\/10.1093\/humupd\/dmt060)"},{"key":"bib26","series-title":"Molecular Reproduction and Development","first-page":"183","article-title":"The uterus is a potential site for anandamide synthesis and hydrolysis: differential profiles of anandamide synthase and hydrolase activities in the mouse uterus during the periimplantation period","volume":"45","author":"Paria","year":"1996","unstructured":"Paria BCDeutsch DDDey SK1996 The uterus is a potential site for anandamide synthesis and hydrolysis: differential profiles of anandamide synthase and hydrolase activities in the mouse uterus during the periimplantation period. Molecular Reproduction and Development 45 183\u2013192. (https:\/\/doi.org\/10.1002\/(SICI)"},{"key":"bib27","series-title":"PNAS","first-page":"4188","article-title":"Changes in anandamide levels in mouse uterus are associated with uterine receptivity for embryo implantation","volume":"94","author":"Schmid","year":"1997","unstructured":"Schmid PCParia BCKrebsbach RJSchmid HHDey SK1997 Changes in anandamide levels in mouse uterus are associated with uterine receptivity for embryo implantation. PNAS 94 4188\u20134192. (https:\/\/doi.org\/10.1073\/pnas.94.8.4188)"},{"key":"bib28","series-title":"Molecular and Cellular Endocrinology","first-page":"S3","article-title":"Aspects of endocannabinoid signaling in periimplantation biology","volume":"286","author":"Sun","year":"2008","unstructured":"Sun XDey SK2008 Aspects of endocannabinoid signaling in periimplantation biology. Molecular and Cellular Endocrinology 286 S3\u2013S11. (https:\/\/doi.org\/10.1016\/j.mce.2008.01.002)"},{"key":"bib29","series-title":"Human Reproduction Update","first-page":"501","article-title":"The role of the endocannabinoid system in gametogenesis, implantation and early pregnancy","volume":"13","author":"Taylor","year":"2007","unstructured":"Taylor AHAng CBell SCKonje JC2007 The role of the endocannabinoid system in gametogenesis, implantation and early pregnancy. Human Reproduction Update 13 501\u2013513. (https:\/\/doi.org\/10.1093\/humupd\/dmm018)"},{"key":"bib30","series-title":"Reproductive Biology and Endocrinology","article-title":"Modulation of the endocannabinoid system in viable and non-viable first trimester pregnancies by pregnancy-related hormones","volume":"9","author":"Taylor","year":"2011","unstructured":"Taylor AHFinney MLam PMKonje JC2011 Modulation of the endocannabinoid system in viable and non-viable first trimester pregnancies by pregnancy-related hormones. Reproductive Biology and Endocrinology 9 152. (https:\/\/doi.org\/10.1186\/1477-7827-9-152)"},{"key":"bib31","series-title":"Placenta","first-page":"579","article-title":"Endocannabinoid system and nitric oxide are involved in the deleterious effects of lipopolysaccharide on murine decidua","volume":"30","author":"Vercelli","year":"2009","unstructured":"Vercelli CAAisemberg JBilli SWolfson MLFranchi AM2009 Endocannabinoid system and nitric oxide are involved in the deleterious effects of lipopolysaccharide on murine decidua. Placenta 30 579\u2013584. (https:\/\/doi.org\/10.1016\/j.placenta.2009.04.003)"},{"key":"bib32","series-title":"Journal of Cell Science","first-page":"1704","article-title":"Integrin clustering enables anandamide-induced Ca2+ signaling in endothelial cells via GPR55 by protection against CB1-receptor-triggered repression","volume":"121","author":"Waldeck-Weiermair","year":"2008","unstructured":"Waldeck-Weiermair MZoratti COsibow KBalenga NGoessnitzer EWaldhoer MMalli RGraier WF2008 Integrin clustering enables anandamide-induced Ca2+ signaling in endothelial cells via GPR55 by protection against CB1-receptor-triggered repression. Journal of Cell Science 121 1704\u20131717. (https:\/\/doi.org\/10.1242\/jcs.020958)"},{"key":"bib33","series-title":"Prostaglandins and Other Lipid Mediators","first-page":"112","article-title":"Biology of endocannabinoid synthesis system","volume":"89","author":"Wang","year":"2009","unstructured":"Wang JUeda N2009 Biology of endocannabinoid synthesis system. Prostaglandins and Other Lipid Mediators 89 112\u2013119. (https:\/\/doi.org\/10.1016\/j.prostaglandins.2008.12.002)"},{"key":"bib34","series-title":"Biology of Reproduction","first-page":"839","article-title":"Stage-specific excitation of cannabinoid receptor exhibits differential effects on mouse embryonic development","volume":"60","author":"Wang","year":"1999","unstructured":"Wang JParia BCDey SKArmant DR1999 Stage-specific excitation of cannabinoid receptor exhibits differential effects on mouse embryonic development. Biology of Reproduction 60 839\u2013844. (https:\/\/doi.org\/10.1095\/biolreprod60.4.839)"},{"key":"bib35","series-title":"Endocrine Reviews","first-page":"427","article-title":"Jekyll and Hyde: two faces of cannabinoid signaling in male and female fertility","volume":"27","author":"Wang","year":"2006","unstructured":"Wang HDey SKMaccarrone M2006 Jekyll and Hyde: two faces of cannabinoid signaling in male and female fertility. Endocrine Reviews 27 427\u2013448. (https:\/\/doi.org\/10.1210\/er.2006-0006)"},{"key":"bib36","series-title":"Analytical Chemistry Insights","first-page":"21","article-title":"Quality issues of research antibodies","volume":"11","author":"Weller","year":"2016","unstructured":"Weller MG2016 Quality issues of research antibodies. Analytical Chemistry Insights 11 21\u201327. (https:\/\/doi.org\/10.4137\/ACI.S31614)"},{"key":"bib37","series-title":"Journal of Biological Chemistry","first-page":"21181","article-title":"Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase-2","volume":"272","author":"Yu","year":"1997","unstructured":"Yu MIves DRamesha CS1997 Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase-2. Journal of Biological Chemistry 272 21181\u201321186. (https:\/\/doi.org\/10.1074\/jbc.272.34.21181)"},{"key":"bib38","series-title":"Nitric Oxide: Biology and Chemistry","first-page":"1","article-title":"Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin beta1 and Talin via the cGMP signalling pathway","volume":"78","author":"Zhan","year":"2018","unstructured":"Zhan RWang FWu YWang YQian WLiu MLiu THe WRen HLuo G2018 Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin beta1 and Talin via the cGMP signalling pathway. Nitric Oxide: Biology and Chemistry 78 1\u201310. (https:\/\/doi.org\/10.1016\/j.niox.2018.04.001)"}],"container-title":["Reproduction and Fertility"],"original-title":[],"link":[{"URL":"https:\/\/raf.bioscientifica.com\/view\/journals\/raf\/2\/2\/RAF-20-0035.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/raf.bioscientifica.com\/downloadpdf\/journals\/raf\/2\/2\/RAF-20-0035.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/raf.bioscientifica.com\/downloadpdf\/journals\/raf\/2\/2\/RAF-20-0035.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:53:12Z","timestamp":1784555592000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/raf\/article\/2\/2\/107-116\/26053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,21]]},"references-count":38,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/raf-20-0035","relation":{},"ISSN":["2633-8386"],"issn-type":[{"value":"2633-8386","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,21]]}}}