{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:49Z","timestamp":1784557129245,"version":"3.55.0"},"reference-count":61,"publisher":"Bioscientifica","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["raf.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,2,16]]},"abstract":"<jats:p>\n                    In the present work, we emphasize the studies about integrins and their receptors in pig placental interface at different times of gestation. Uterine placental interface (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200924) of 17, 30, 60 and 70 days of gestation (dg) and non-pregnant uterus (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20094) of crossbred sows were used. The presence of \u03b1v\u03b23 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and \u03b15\u03b21 (ITGA5 (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) integrins, and their ligands fibronectin (FN) and osteopontin (OPN\/ SPP1), was detected by immunohistochemistry, and the immunolabeled area percentage (IAP) and the optical density (OD) were determined. The integrins and its ligands analyzed have presented peaks of expression in early and mid-gestation, both in IAP and in the OD area, decreasing at 70 dg. These temporal changes showed us that the molecules studied in this work participate in embryo\/feto\u2013maternal attachment, variably. Besides, we found a significant correlation both in the intensity and in the extension of immunostaining for trophoblastic FN and endometrial \u03b1v\u03b23, and trophoblastic OPN and endometrial \u03b15\u03b21, throughout the entire pig pregnancy. At late gestation, there is notable placental remodeling with subsequent removal or renewal of folds at the uterine\u2013placental interface that results in the loss of focal adhesions. The decrease of the expression of some integrins and their ligands in late gestation, particularly at 70 dg, would demonstrate that there would be other adhesion molecules and other ligands that could be participating in the establishment of the maternal\u2013fetal interface.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>Lay summary<\/jats:title>\n                    <jats:p>To carry a successful pregnancy, the formation of the placenta in pigs depends on adhesion molecules. Some of these molecules called integrins bind to other molecules such as fibronectin (FN) and osteopontin (OPN\/SPP1). The variation in those molecule amounts during gestation would indicate which molecule is participating and what role it plays in pregnancy. We worked with pig placentas of early, mid- and late- gestation and non-pregnant uteruses. \u03b1v\u03b23 (ITGAV (integrin subunit alpha V) ITGB3 (integrin subunit beta 3)) and \u03b15\u03b21 (ITGA5 (integrin subunit alpha 5) ITGB1 (integrin subunit beta 1)) integrins, FN and OPN were found until mid-gestation but not at late gestation, suggesting that other types of molecules have a role in the last period of gestation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/raf-22-0079","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T13:26:30Z","timestamp":1676553990000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-13","source":"Crossref","is-referenced-by-count":1,"title":["Are integrins and ligands correlated at pig placental interface during pregnancy?"],"prefix":"10.1530","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9511-3045","authenticated-orcid":true,"given":"Velez","family":"Carolina","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science, National University of La Pampa (UNLPam), Argentina"},{"name":"National Scientific and Technical Research Council of Argentina (CONICET), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clauzure","family":"Mariangeles","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, National University of La Pampa (UNLPam), Argentina"},{"name":"National Scientific and Technical Research Council of Argentina (CONICET), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Williamson","family":"Delia","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, National University of La Pampa (UNLPam), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garcia","family":"Monica","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, National University of La Pampa (UNLPam), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koncurat","family":"Mirta","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, National University of La Pampa (UNLPam), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbeito","family":"Claudio","sequence":"additional","affiliation":[{"name":"National Scientific and Technical Research Council of Argentina (CONICET), Argentina"},{"name":"Laboratory of Descriptive, Comparative and Experimental Histology and Embriology (LHYEDEC). Faculty of Veterinary Science, National University of La Plata (UNLP), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Endocrinology","first-page":"4306","article-title":"Up-regulation of alpha5-integrin by E-cadherin loss in hypoxia and its key role in the migration of extravillous trophoblast cells during early implantation","volume":"150","author":"Arimoto-Ishida","year":"2009","unstructured":"Arimoto-Ishida ESakata MSawada KNakayama MNishimoto FMabuchi STakeda TYamamoto TIsobe AOkamoto Y, 2009Up-regulation of alpha5-integrin by E-cadherin loss in hypoxia and its key role in the migration of extravillous trophoblast cells during early implantation. Endocrinology1504306\u20134315. 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