{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:49Z","timestamp":1784557129867,"version":"3.55.0"},"reference-count":5,"publisher":"Bioscientifica","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["raf.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Lay summary<\/jats:title>\n                    <jats:p>Friction caused by blood flowing across cells that line blood vessels (endothelial cells) activates sensors of mechanical force. This produces nitric oxide (NO) which widens placental blood vessels, enabling more blood flow to the baby. This study sought to determine whether the mechanical sensor, Piezo1, is important for NO production in fetoplacental endothelial cells (FpECs) and whether the steps in this pathway are different in small for gestational age (SGA) babies, where placental blood flow is often altered. We showed that in healthy FpECs, blood flow increased NO signalling. We suggest that in SGA babies, FpECs have an increase in baseline levels of NO signalling, suggestive of a compensatory drive. Treating healthy and SGA cells with a Piezo1 chemical activator, Yoda1, upregulated NO signalling. This shows that Piezo1 is linked to NO and that in SGA, FpECs have the capacity to further increase NO. Further research will establish whether Piezo1 enhancement leads to increased blood flow in the placenta. If so, Piezo1 could be a new target for developing treatments to prevent poor growth of babies in the womb.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/raf-22-0100","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T13:23:10Z","timestamp":1671628990000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-13","source":"Crossref","is-referenced-by-count":8,"title":["Nitric oxide synthase phosphorylation in fetoplacental endothelium is enhanced by agonism of Piezo1 mechanosensor in small for gestational age babies"],"prefix":"10.1530","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1109-1396","authenticated-orcid":true,"given":"L C","family":"Morley","sequence":"first","affiliation":[{"name":"Leeds Institute of Cardiovascular and Metabolic Medicine, LIGHT laboratories, University of Leeds, UK"},{"name":"Academic Department of Obstetrics and Gynaecology, Level 9 Worsley Building, School of Medicine, University of Leeds, Leeds, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Debant","sequence":"additional","affiliation":[{"name":"Leeds Institute of Cardiovascular and Metabolic Medicine, LIGHT laboratories, University of Leeds, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H J","family":"Gaunt","sequence":"additional","affiliation":[{"name":"Leeds Institute of Cardiovascular and Metabolic Medicine, LIGHT laboratories, University of Leeds, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N A B","family":"Simpson","sequence":"additional","affiliation":[{"name":"Academic Department of Obstetrics and Gynaecology, Level 9 Worsley Building, School of Medicine, University of Leeds, Leeds, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D J","family":"Beech","sequence":"additional","affiliation":[{"name":"Leeds Institute of Cardiovascular and Metabolic Medicine, LIGHT laboratories, University of Leeds, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Seminars in Fetal and Neonatal Medicine","first-page":"119","article-title":"Screening for fetal growth restriction and placental insufficiency","volume":"23","author":"Audette","year":"2018","unstructured":"Audette MCKingdom JC2018Screening for fetal growth restriction and placental insufficiency. Seminars in Fetal and Neonatal Medicine23119\u2013125. (https:\/\/doi.org\/10.1016\/j.siny.2017.11.004)"},{"key":"bib2","series-title":"American Journal of Obstetrics and Gynecology","first-page":"583","article-title":"Nitric oxide is involved in flow-induced dilatation of isolated human small fetoplacental arteries","volume":"174","author":"Learmont","year":"1996","unstructured":"Learmont JGPoston L1996Nitric oxide is involved in flow-induced dilatation of isolated human small fetoplacental arteries. American Journal of Obstetrics and Gynecology174583\u2013588. (https:\/\/doi.org\/10.1016\/s0002-9378(9670432-5)"},{"key":"bib3","series-title":"Nature","first-page":"279","article-title":"Piezo1 integration of vascular architecture with physiological force","volume":"515","author":"Li","year":"2014","unstructured":"Li JHou BTumova SMuraki KBruns ALudlow MJSedo AHyman AJMcKeown LYoung RS2014Piezo1 integration of vascular architecture with physiological force. Nature515279\u2013282. (https:\/\/doi.org\/10.1038\/nature13701)"},{"key":"bib4","series-title":"Molecular Human Reproduction","first-page":"510","article-title":"Piezo1 channels are mechanosensors in human fetoplacental endothelial cells","volume":"24","author":"Morley","year":"2018","unstructured":"Morley LCShi JGaunt HJHyman AJWebster PJWilliam CForbes KWalker JJSimpson NABBeech DJ2018Piezo1 channels are mechanosensors in human fetoplacental endothelial cells. Molecular Human Reproduction24510\u2013520. (https:\/\/doi.org\/10.1093\/molehr\/gay033)"},{"key":"bib5","series-title":"International Journal of Developmental Biology","first-page":"331","article-title":"Shear stress regulation of nitric oxide production in uterine and placental artery endothelial cells: experimental studies and haemodynamic models of shear stresses on endothelial cells","volume":"54","author":"Sprague","year":"2010","unstructured":"Sprague BChesler NCMagness RR2010Shear stress regulation of nitric oxide production in uterine and placental artery endothelial cells: experimental studies and haemodynamic models of shear stresses on endothelial cells. International Journal of Developmental Biology54331\u2013339. (https:\/\/doi.org\/10.1387\/ijdb.082832bs)"}],"container-title":["Reproduction and Fertility"],"original-title":[],"link":[{"URL":"https:\/\/raf.bioscientifica.com\/view\/journals\/raf\/4\/1\/RAF-22-0100.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/raf.bioscientifica.com\/downloadpdf\/journals\/raf\/4\/1\/RAF-22-0100.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/raf.bioscientifica.com\/downloadpdf\/journals\/raf\/4\/1\/RAF-22-0100.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:53:48Z","timestamp":1784555628000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/raf\/article\/26152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,1]]},"references-count":5,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/raf-22-0100","relation":{},"ISSN":["2633-8386"],"issn-type":[{"value":"2633-8386","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,1]]}}}