{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:20:58Z","timestamp":1791555658713,"version":"4.3.4"},"reference-count":36,"publisher":"Bioscientifica","issue":"3","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["raf.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7,10]]},"abstract":"\n Graphical abstract<\/jats:title>\n \n \n <\/jats:p>\n <\/jats:sec>\n \n Abstract<\/jats:title>\n \n Reactive oxygen species (ROS) are a by-product of the activity of cytochrome P450 steroidogenic enzymes. Antioxidant enzymes protect against ROS damage. To identify if any particular antioxidant enzyme is used to protect against ROS produced by granulosa cells as follicles enlarge and produce oestradiol, we measured in the bovine granulosa cells the expression of two steroidogenic enzymes (\n CYP11A1<\/jats:italic>\n ,\n CYP19A1<\/jats:italic>\n ), important for progesterone and oestradiol production. We also measured the expression of the members (\n FDXR<\/jats:italic>\n ,\n FDX1<\/jats:italic>\n ,\n POR<\/jats:italic>\n ) of their electron transport chains (ETC). We measured antioxidant enzymes (\n GPX<\/jats:italic>\n s\n 1<\/jats:italic>\n \u2013\n 8<\/jats:italic>\n ,\n CAT<\/jats:italic>\n ,\n SOD<\/jats:italic>\n s\n 1<\/jats:italic>\n and\n 2<\/jats:italic>\n ,\n PRDX<\/jats:italic>\n s\n 1<\/jats:italic>\n \u2013\n 6<\/jats:italic>\n ,\n GSR<\/jats:italic>\n ,\n TXN<\/jats:italic>\n ,\n TXNRD<\/jats:italic>\n s\n 1<\/jats:italic>\n \u2013\n 3<\/jats:italic>\n ). Since selenium is an active component of GPXs, the selenium-uptake receptors (\n LRP<\/jats:italic>\n s\n 2<\/jats:italic>\n and\n 8<\/jats:italic>\n ) were measured. Only the selenium-dependent\n GPX1<\/jats:italic>\n showed the same increase in expression as the steroidogenic enzymes did with increasing follicle size.\n GPX4<\/jats:italic>\n and\n PRDX2\/6<\/jats:italic>\n decreased with follicle size, whereas\n SOD1\/2<\/jats:italic>\n ,\n CAT<\/jats:italic>\n ,\n GSR<\/jats:italic>\n , and\n TXNRD3<\/jats:italic>\n were lowest at the intermediate sizes. The other antioxidant enzymes were unchanged or expressed at low levels. The expression of the selenium-uptake receptor\n LRP8<\/jats:italic>\n also increased significantly with follicle size. Correlation analysis revealed statistically significant and strongly positive correlations of the steroidogenic enzymes and their ETCs with both\n GPX1<\/jats:italic>\n and\n LRP8<\/jats:italic>\n . These results demonstrate a relationship between the expression of genes involved in steroidogenesis and selenium-containing antioxidant defence mechanisms. They suggest that during the late stages of folliculogenesis, granulosa cells are dependent on sufficient expression of\n GPX1<\/jats:italic>\n and the selenium transporter\n LRP8<\/jats:italic>\n to counteract increasing ROS levels caused by the production of steroid hormones.\n <\/jats:p>\n <\/jats:sec>\n \n Lay summary<\/jats:title>\n In the ovary, eggs are housed in follicles which contain the cells that produce oestrogen in the days leading up to ovulation of the egg. Oestrogen is produced by the action of enzymes. However, some of these enzymes also produce by-products called reactive oxygen species (ROS). These are harmful to eggs. Fortunately, cells have protective antioxidant enzymes that can neutralise ROS. This study was interested in which particular antioxidant enzyme(s) might be involved in neutralising the ROS in follicle cells. It was found that only one antioxidant enzyme, GPX1, appeared to be co-regulated with the enzymes that produce oestrogen and progesterone in the follicular cells. GPX1 contains the essential mineral selenium. In summary, this study has identified which antioxidant appears to be involved in neutralising ROS in the days leading to ovulation. It highlights the importance of selenium in the diet.<\/jats:p>\n <\/jats:sec>","DOI":"10.1530\/raf-23-0074","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T12:23:31Z","timestamp":1720700611000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-13","source":"Crossref","is-referenced-by-count":4,"title":["Expression levels of the selenium-uptake receptor LRP8, the antioxidant selenoprotein GPX1 and steroidogenic enzymes correlate in granulosa cells"],"prefix":"10.1530","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5023-2668","authenticated-orcid":true,"given":"Katja","family":"Hummitzsch","sequence":"first","affiliation":[{"name":"Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine E","family":"Kelly","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Hatzirodos","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy M","family":"Bonner","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Tang","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh H","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Adelaide, South Australia, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2139-2969","authenticated-orcid":true,"given":"Raymond J","family":"Rodgers","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Biomedicine, The University of Adelaide, South Australia, Australia."},{"name":"Adelaide Health and Medical Sciences Building, The University of Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Journal of Assisted Reproduction and Genetics","first-page":"483","article-title":"The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media","volume":"25","author":"Abedelahi","year":"2008","unstructured":"Abedelahi ASalehnia M & Allameh AA2008The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media. 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