{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:27:35Z","timestamp":1767814055343,"version":"3.49.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To assess the role of protein kinase C\u03b2 (PKC\u03b2) in human myometrial contractions during pregnancy, we evaluated the effect of a PKC\u03b2 inhibitor (LY333531) on the pregnant and nonpregnant myometrial contractions and compared the level of PKC\u03b2 in the pregnant myometrium with that in the nonpregnant myometrium. The effects of LY333531 on the myometrial contractions were examined by measuring contractile activity (frequency and amplitude). PKC\u03b2 in human myometrium was assessed at mRNA level using real-time PCR method. The characteristics of contractile activity were different between the pregnant and the nonpregnant myometrium. The amplitude of rhythmic contractions in the preterm and term myometrium was increased 2- to 2.5-fold when compared with that in the nonpregnant myometrium, but the frequency of rhythmic contractions was decreased by about half. LY333531 (10\u22126 M) reduced the increased amplitude in the preterm and term myometrium by about 50%, and the inhibitory effects of LY333531 in the pregnant myometrium were significantly greater than that in the nonpregnant myometrium (about 50 vs 25%). However, the frequency in the pregnant and nonpregnant myometrium was not influenced by LY333531. Real-time PCR revealed a significant, five- to sevenfold increase in the expression of PKC\u03b2 mRNA in the preterm and term myometrium when compared with the nonpregnant myometrium. These findings suggest that the increased amplitude of human myometrial contractions during pregnancy is related to the increased level of PKC\u03b2. A PKC\u03b2 inhibitor may reduce preterm uterine contractions and prevent preterm delivery.<\/jats:p>","DOI":"10.1530\/rep-06-0041","type":"journal-article","created":{"date-parts":[[2007,5,15]],"date-time":"2007-05-15T11:51:46Z","timestamp":1179229906000},"page":"797-806","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":10,"title":["Role of protein kinase C\u03b2 in rhythmic contractions of human pregnant myometrium"],"prefix":"10.1093","volume":"133","author":[{"given":"Katsuhiko","family":"Yasuda","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Nakamoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, 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