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In this study, we examined the involvement of carnitine\/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na+-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with Km values of 23.6 and 6.57 \u03bcM for carnitine and acetylcarnitine respectively in the presence of Na+. Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na+-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.<\/jats:p>","DOI":"10.1530\/rep-06-0173","type":"journal-article","created":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T11:29:18Z","timestamp":1193398158000},"page":"651-658","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":35,"title":["Transport of carnitine and acetylcarnitine by carnitine\/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa"],"prefix":"10.1093","volume":"134","author":[{"given":"Daisuke","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Division of Pharmaceutical Sciences, Department of Molecular Biopharmaceutics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikumi","family":"Tamai","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimichi","family":"Sai","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Sciences, Department of Molecular Biopharmaceutics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Sciences, Department of Molecular Biopharmaceutics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiko","family":"Wakayama","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, Takara-machi, Kanazawa 920-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuto","family":"Kido","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Sciences, Department of Molecular Biopharmaceutics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-ichi","family":"Nezu","sequence":"additional","affiliation":[{"name":"Chugai Pharmaceutical Co. 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