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The mechanism(s) by which a mammalian egg regulates the establishment of the membrane block to polyspermy is largely unknown. Since Ca2+ signaling regulates several egg activation events, this study investigates how sperm-induced Ca2+ transients affect the membrane block to polyspermy, building on our previous work (Biology of Reproduction 67:1342). We demonstrate that mouse eggs that experience only one sperm-induced Ca2+ transient establish a membrane block that is less effective, than in eggs that experience normal sperm-induced Ca2+ transients but that is more effective than in eggs with completely suppressed [Ca2+]cyt increases. Sperm-induced increases in [Ca2+]cyt regulate the timing of membrane block establishment, as this block is established more slowly in eggs that experience one or no sperm-induced Ca2+ transients. Finally, our studies produce the intriguing discovery that there is also a Ca2+-independent event that is associated with fertilization in the pathway leading to membrane block establishment. Taken together, these data indicate that Ca2+ plays a role in facilitating membrane block establishment by regulating the timing with which this change in egg membrane function occurs, and also that the membrane block differs from other post-fertilization egg activation responses as Ca2+ is not the only stimulus. The membrane block to polyspermy in mammalian eggs is likely to be the culmination of multiple post-fertilization events that together modify the egg membrane\u2019s receptivity to sperm.<\/jats:p>","DOI":"10.1530\/rep-06-0304","type":"journal-article","created":{"date-parts":[[2007,2,16]],"date-time":"2007-02-16T11:48:14Z","timestamp":1171626494000},"page":"383-393","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":40,"title":["Establishment of the mammalian membrane block to polyspermy: evidence for calcium-dependent and -independent regulation"],"prefix":"10.1093","volume":"133","author":[{"given":"Allison J","family":"Gardner","sequence":"first","affiliation":[{"name":"Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Room W3606, 615 N. 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