{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:18:02Z","timestamp":1767849482895,"version":"3.49.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study investigated cortisol inactivation by 11\u03b2-hydroxysteroid dehydrogenase (11\u03b2 HSD) enzymes in porcine granulosa cells from antral follicles at different developmental stages and in ovarian cysts. In granulosa cells, cortisol oxidation increased threefold with antral follicle diameter (P &amp;lt; 0.001). This trend was paralleled by a threefold increase in NADP+-dependent 11\u03b2-dehydrogenase activity in granulosa cell homogenates with follicle diameter. Intact granulosa cells from ovarian cysts exhibited significantly lower enzyme activities than cells from large antral follicles. Neither intact cells norcell homogenates displayed net 11-ketosteroid reductase activities. Since porcine follicular fluid (FF) from large antral follicles and ovarian cysts contains hydrophobic inhibitors of glucocorticoid metabolism by type 1 11\u03b2 HSD, this studyalso investigated whether levels of 11\u03b2 HSD inhibitors changed during follicle growth and could affect cortisol metabolism in granulosa cells. The extent of inhibition of 11\u03b2 HSD1 activity in rat kidney homogenates decreased progressively from 50 \u00b1 8% inhibition by FF from small antral follicles (P &amp;lt; 0.001) to 23 \u00b1 6% by large antral FF (P &amp;lt; 0.05). Cyst fluid inhibited 11\u03b2 HSD1 activity by 59 \u00b1 4% (P &amp;lt; 0.001). Likewise, net cortisol oxidation in granulosa cells was significantly decreased by large antral FF (35\u201348% inhibition, P &amp;lt; 0.05) and cyst fluid (45\u201375% inhibition, P &amp;lt; 0.01). We conclude that inactivation of cortisol by 11\u03b2 HSD enzymes in porcine granulosa cells increases with follicle development but is significantly decreased in ovarian cysts. Moreover, changes in ovarian cortisol metabolism are accompanied by corresponding changes in the levels of paracrine inhibitors of 11\u03b2 HSD1 within growing ovarian follicles and cysts, implicating cortisol in follicle growth and cyst development.<\/jats:p>","DOI":"10.1530\/rep-07-0003","type":"journal-article","created":{"date-parts":[[2007,7,16]],"date-time":"2007-07-16T12:53:09Z","timestamp":1184590389000},"page":"1149-1158","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":17,"title":["Implication of cortisol and 11\u03b2-hydroxysteroid dehydrogenase enzymes in the development of porcine (\n                    <i>Sus scrofa domestica<\/i>\n                    ) ovarian follicles and cysts"],"prefix":"10.1093","volume":"133","author":[{"given":"Neera","family":"Sunak","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daphne F","family":"Green","sequence":"additional","affiliation":[{"name":"Institute of Zoology, Zoological Society of London, Regent\u2019s Park, London NW1 4RY, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lalantha R","family":"Abeydeera","sequence":"additional","affiliation":[{"name":"Genus Plc, 3033 Nashville Road, Franklin, Kentucky 42135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa M","family":"Thurston","sequence":"additional","affiliation":[{"name":"Reproduction and Development Group, Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony E","family":"Michael","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK"},{"name":"Division of Clinical Developmental Sciences, Academic Section of Obstetrics and Gynaecology, St George\u2019s, University of London, Cranmer Terrace, Tooting SW17 0RE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2007,6,1]]},"reference":[{"key":"2026010713120183800_AGARWAL-ETAL-1989","doi-asserted-by":"crossref","first-page":"18939","DOI":"10.1016\/S0021-9258(19)47248-7","volume":"264","year":"1989","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713120183800_AGARWAL-ETAL-1994","doi-asserted-by":"crossref","first-page":"25959","DOI":"10.1016\/S0021-9258(18)47142-6","volume":"269","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713120183800_ALBISTON-ETAL-1994","doi-asserted-by":"crossref","first-page":"R11","DOI":"10.1016\/0303-7207(94)90176-7","volume":"105","year":"1994","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010713120183800_ATANASOV-ETAL-2004","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.febslet.2004.06.065","volume":"571","year":"2004","journal-title":"FEBS Letters"},{"key":"2026010713120183800_BANHEGYI-ETAL-2004","doi-asserted-by":"crossref","first-page":"27017","DOI":"10.1074\/jbc.M404159200","volume":"279","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713120183800_BROWN-ETAL-1993","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1210\/endo.132.6.8504762","volume":"132","year":"1993","journal-title":"Endocrinology"},{"key":"2026010713120183800_CALDER-ETAL-2001","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1095\/biolreprod65.2.471","volume":"65","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010713120183800_CONLEY-ETAL-1994","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1095\/biolreprod51.4.655","volume":"51","year":"1994","journal-title":"Biology of Reproduction"},{"key":"2026010713120183800_DRAPER-ETAL-2003","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1038\/ng1214","volume":"34","year":"2003","journal-title":"Nature Genetics"},{"key":"2026010713120183800_GAO-ETAL-1997","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1210\/endo.138.1.4837","volume":"138","year":"1997","journal-title":"Endocrinology"},{"key":"2026010713120183800_GE-AND-HARDY-2000","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/j.1939-4640.2000.tb02108.x","volume":"21","year":"2000","journal-title":"Journal of Andrology"},{"key":"2026010713120183800_GE-ETAL-2005","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1210\/en.2005-0046","volume":"146","year":"2005","journal-title":"Endocrinology"},{"key":"2026010713120183800_GUTHRIE-ETAL-1995","doi-asserted-by":"crossref","first-page":"2834","DOI":"10.2527\/1995.7392834x","volume":"73","year":"1995","journal-title":"Journal of Animal Science"},{"key":"2026010713120183800_HUGHES-AND-GOROSPE-1991","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1210\/endo-129-5-2415","volume":"129","year":"1991","journal-title":"Endocrinology"},{"key":"2026010713120183800_HULT-ETAL-2004","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1007\/s00018-003-3476-y","volume":"61","year":"2004","journal-title":"Cellular and Molecular Life Sciences"},{"key":"2026010713120183800_JAMIESON-ETAL-1995","doi-asserted-by":"crossref","first-page":"4754","DOI":"10.1210\/endo.136.11.7588203","volume":"136","year":"1995","journal-title":"Endocrinology"},{"key":"2026010713120183800_KESLER-AND-GARVERICK-1982","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2527\/jas1982.5551147x","volume":"55","year":"1982","journal-title":"Journal of Animal Science"},{"key":"2026010713120183800_KNOX-2005","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.domaniend.2005.02.025","volume":"29","year":"2005","journal-title":"Domestic Animal Endocrinology"},{"key":"2026010713120183800_LAKSHMI-AND-MONDER-1988","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.1210\/endo-123-5-2390","volume":"123","year":"1988","journal-title":"Endocrinology"},{"key":"2026010713120183800_LATIF-ETAL-2005","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.mce.2005.08.006","volume":"243","year":"2005","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010713120183800_LOW-ETAL-1994","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1677\/jme.0.0130167","volume":"13","year":"1994","journal-title":"Journal of Molecular Endocrinology"},{"key":"2026010713120183800_MAXSON-ETAL-1985","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1095\/biolreprod33.2.495","volume":"33","year":"1985","journal-title":"Biology of Reproduction"},{"key":"2026010713120183800_MCCORMICK-ETAL-2006","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1074\/jbc.M506026200","volume":"281","year":"2006","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713120183800_MERCER-AND-KROZOWSKI-1992","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1210\/endo.130.1.1727721","volume":"130","year":"1992","journal-title":"Endocrinology"},{"key":"2026010713120183800_MICHAEL-ETAL-1997","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0303-7207(97)00118-4","volume":"132","year":"1997","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010713120183800_MICHAEL-ETAL-2003","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1530\/rep.0.1260425","volume":"126","year":"2003","journal-title":"Reproduction"},{"key":"2026010713120183800_MONDER-AND-LAKSHMI-1990","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1210\/endo-126-5-2435","volume":"126","year":"1990","journal-title":"Endocrinology"},{"key":"2026010713120183800_NARAY-FEJES-TOTH-ETAL-1991","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1210\/endo-129-1-17","volume":"129","year":"1991","journal-title":"Endocrinology"},{"key":"2026010713120183800_PALLIKAROS-ETAL-1995","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0303-7207(95)03642-K","volume":"114","year":"1995","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010713120183800_RICKETTS-ETAL-1998","first-page":"1325","volume":"83","year":"1998","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"2026010713120183800_ROBINZON-AND-PROUGH-2005","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.abb.2005.07.010","volume":"442","year":"2005","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"2026010713120183800_ROBINZON-ETAL-2003","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0003-9861(03)00056-0","volume":"412","year":"2003","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"2026010713120183800_SASSON-ETAL-2001","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1210\/endo.142.2.7942","volume":"142","year":"2001","journal-title":"Endocrinology"},{"key":"2026010713120183800_SCHOONMAKER-AND-ERICKSON-1983","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1210\/endo-113-4-1356","volume":"113","year":"1983","journal-title":"Endocrinology"},{"key":"2026010713120183800_SCHWEIZER-ETAL-2004","doi-asserted-by":"crossref","first-page":"18415","DOI":"10.1074\/jbc.M313615200","volume":"279","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713120183800_SECKL-AND-WALKER-2001","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1210\/endo.142.4.8114","volume":"142","year":"2001","journal-title":"Endocrinology"},{"key":"2026010713120183800_SOUNESS-AND-MORRIS-1996","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1161\/01.HYP.27.3.421","volume":"27","year":"1996","journal-title":"Hypertension"},{"key":"2026010713120183800_SOUNESS-ETAL-1995","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1210\/endo.136.4.7895695","volume":"136","year":"1995","journal-title":"Endocrinology"},{"key":"2026010713120183800_SUN-ETAL-1998","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1095\/biolreprod58.6.1379","volume":"58","year":"1998","journal-title":"Biology of Reproduction"},{"key":"2026010713120183800_TANNIN-ETAL-1991","doi-asserted-by":"crossref","first-page":"16653","DOI":"10.1016\/S0021-9258(18)55351-5","volume":"266","year":"1991","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713120183800_TETSUKA-ETAL-2003","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1677\/joe.0.1770445","volume":"177","year":"2003","journal-title":"Journal of Endocrinology"},{"key":"2026010713120183800_THURSTON-ETAL-2002","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1530\/rep.0.1240801","volume":"124","year":"2002","journal-title":"Reproduction"},{"key":"2026010713120183800_THURSTON-ETAL-2003A","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1095\/biolreprod.102.012096","volume":"68","year":"2003","journal-title":"Biology of Reproduction"},{"key":"2026010713120183800_THURSTON-ETAL-2003B","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1677\/joe.0.1780127","volume":"178","year":"2003","journal-title":"Journal of Endocrinology"},{"key":"2026010713120183800_TILLY-ETAL-1992","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1210\/endo.131.4.1396312","volume":"131","year":"1992","journal-title":"Endocrinology"},{"key":"2026010713120183800_YANG-ETAL-1999","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1095\/biolreprod60.4.929","volume":"60","year":"1999","journal-title":"Biology of Reproduction"},{"key":"2026010713120183800_YONG-ETAL-2000","first-page":"4728","volume":"85","year":"2000","journal-title":"Journal of Clinical Endocrinology and Metabolism"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/133\/6\/1331149.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rep.bioscientifica.com\/downloadpdf\/journals\/rep\/133\/6\/1331149.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/133\/6\/1149\/64608527\/1331149.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/133\/6\/1149\/64608527\/1331149.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:23:19Z","timestamp":1767810199000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/133\/6\/1149\/8252525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,1]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,6,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-07-0003","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,6,1]]},"published":{"date-parts":[[2007,6,1]]}}}