{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:13:45Z","timestamp":1787022825923,"version":"build-2736575974"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fetal Leydig cells and fetal adrenocortical cells may share a common progenitor cell. Both cell types show several similarities, particularly in relation to their primary steroidogenic function. Differences in steroid secretion are largely due to the expression of 21-hydroxylase (CYP21A1) and 11\u03b2-hydroxylase (CYP11B1) activity in the adrenal. To determine whether expression of these enzymes defines a clear difference between adrenocortical and Leydig cells, or is further evidence of a link between the cell types, we have measured Cyp21a1 and Cyp11b1 expression and related enzyme activity in the fetal testis. Expression of both Cyp21a1 and Cyp11b1 was clearly detectable in the fetal testis by RT-PCR and Southern blotting. Real-time PCR studies showed that Cyp11b1 was expressed only in the fetal\/neonatal testis with no expression in the pubertal or post-pubertal animal. Cyp21a1 was also predominantly expressed in the fetal testis although some lower expression was also seen in the adult. Expression of Cyp21a1 and Cyp11b1 in neonatal testicular cells was unaffected by incubation in vitro with human chorionic gonadotrophin or ACTH. Using immunohistochemistry, CYP21A1 was localised to a subset of interstitial steroidogenic cells in the fetal testis although CYP11B1 was not detectable. Incubation studies showed that 21-hydroxylase activity was present in the tissue although 11\u03b2-hydroxylase activity could not be detected. Results indicate that a subpopulation of steroidogenic cells in the fetal testis express Cyp21a1 and show 21-hydroxylase activity. This may provide further evidence of a link between fetal Leydig cells and adrenocortical cells but does not discount the possibility that these steroidogenic cells represent \u2018ectopic\u2019 adrenal cells.<\/jats:p>","DOI":"10.1530\/rep-07-0133","type":"journal-article","created":{"date-parts":[[2007,9,21]],"date-time":"2007-09-21T10:18:44Z","timestamp":1190369924000},"page":"585-591","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":30,"title":["Expression of\n                    <i>Cyp21a1<\/i>\n                    and\n                    <i>Cyp11b1<\/i>\n                    in the fetal mouse testis"],"prefix":"10.1093","volume":"134","author":[{"given":"Liangbiao","family":"Hu","sequence":"first","affiliation":[{"name":"Division of Cell Sciences, University of Glasgow Veterinary School, Institute of Comparative Medicine, Bearsden Road, Glasgow G61 1QH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Division of Cell Sciences, University of Glasgow Veterinary School, Institute of Comparative Medicine, Bearsden Road, Glasgow G61 1QH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Johnston","sequence":"additional","affiliation":[{"name":"Division of Cell Sciences, University of Glasgow Veterinary School, Institute of Comparative Medicine, Bearsden Road, Glasgow G61 1QH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"King","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Molecular Endocrinology Centre, Bart\u2019s and The London, Queen Mary, University of London, London EC1M 6BQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter 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