{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:52:27Z","timestamp":1791197547544,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Successful fertilization requires gametes to complete several stages, beginning with maturation and transport along the male and female reproductive tracts and ending with the interaction between the sperm and the egg. This last step involves sperm\u2013egg adhesion and membrane fusion. ADAMs (disintegrin and metalloprotease domain proteins) are a family of membrane-anchored glycoproteins that are thought to play diverse roles in cell\u2013cell adhesion through their interaction with integrins. This study analyzes the presence, location, processing, and possible role of ADAM15 in mouse sperm. The presence of ADAM15 in mouse spermatozoa was detected by Western blotting, which revealed that ADAM15 is post-translationally processed, during epididymal sperm maturation and the acrosome reaction. The 35\u200akDa antigen present in the acrosome-reacted sperm is the last proteolytic product of the 110\/75\u200akDa ADAM15 found in non-capacitated sperm. This 35\u200akDa protein contains the disintegrin domain. By indirect immunofluorescence, ADAM15 was identified in the acrosomal region and along the flagellum of mouse spermatozoa. In acrosome-reacted sperm, ADAM15 was lost from the acrosomal region, but remained diffusely distributed throughout the head and flagellum. Furthermore, the ADAM15 disintegrin domain (RPPTDDCDLPEF) partially inhibited fusion and almost completely inhibited sperm\u2013oolemma adhesion. In conclusion, our data indicate that ADAM15 is present in the testis and in spermatozoa from the caput, corpus, and cauda epididymis, as well as in non-capacitated and acrosome-reacted gametes. Results also indicate that ADAM15 is processed during epididymal maturation and acrosome reaction and that it may play a role during sperm\u2013egg binding.<\/jats:p>","DOI":"10.1530\/rep-07-0300","type":"journal-article","created":{"date-parts":[[2008,4,11]],"date-time":"2008-04-11T11:48:19Z","timestamp":1207914499000},"page":"41-51","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":24,"title":["Presence, processing, and localization of mouse ADAM15 during sperm maturation and the role of its disintegrin domain during sperm\u2013egg binding"],"prefix":"10.1093","volume":"136","author":[{"given":"Karina","family":"Past\u00e9n-Hidalgo","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaura","family":"Hern\u00e1ndez-Rivas","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Lilia","family":"Roa-Espitia","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"S\u00e1nchez-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Mart\u00ednez-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma Olivia","family":"Monrroy","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique O","family":"Hern\u00e1ndez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. M\u00e9xico and 4Posgrado en Ciencias Biol\u00f3gicas Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), PC 04510, M\u00e9xico, DF, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adela","family":"M\u00fajica","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular and 2Departamento de Biomedicina Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional No. 2508 (CINVESTAV-IPN), PC 07360, M\u00e9xico, DF, M\u00e9xico 3Area Acad\u00e9mica de Medicina Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, PC 42090, Pachuca, Hgo. 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