{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:48:33Z","timestamp":1790768913215,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["reproduction-online.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the present study, the prevalence of chromatin instability in the fertilizing-competent sperm population in the porcine oviduct in vivo was examined through qualitative analysis of the chromatin structure status of accessory boar sperm found in in vivo-derived embryos. The binding of chromatin-unstable sperm to oviductal epithelium in vitro was also studied. To examine the sperm chromatin state, a modified fluorescence microscopic sperm chromatin structure assay was used. Among a population of 173 fertile boars, individuals were selected for according to their chromatin status: 25 animals showed more than 5% of chromatin-unstable sperm in their ejaculates, and 7 showed consistently elevated percentages of chromatin-unstable sperm in three successively collected semen samples. A positive correlation was found between incidence of chromatin instability and attached cytoplasmic droplets (r=0.44, P&amp;lt;0.01). Analyses of accessory spermatozoa from in vivo-derived embryos demonstrated that the proportion of chromatin-unstable sperm was significantly (P&amp;lt;0.05) reduced in the population of fertilizing-competent sperm in the oviduct compared with the inseminated sperm. Populations of sperm bound to the oviduct in vitro had significantly (P&amp;lt;0.05) lower percentages of chromatin instability than in the original diluted semen sample. In conclusion, numbers of sperm with unstable chromatin are reduced in the oviductal sperm reservoir, possibly because of associated changes in the plasma membrane that prevent sperm from binding to the oviductal epithelium. We conclude that in vivo the likelihood that sperm with unstable chromatin will reach the egg and fertilize it is low.<\/jats:p>","DOI":"10.1530\/rep-07-0333","type":"journal-article","created":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T20:47:28Z","timestamp":1206564448000},"page":"461-470","update-policy":"https:\/\/doi.org\/10.1530\/rep\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":45,"title":["Chromatin-unstable boar spermatozoa have little chance of reaching oocytes\n                    <i>in vivo<\/i>"],"prefix":"10.1093","volume":"135","author":[{"given":"Florencia","family":"Ard\u00f3n","sequence":"first","affiliation":[{"name":"Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants 2Clinic for Cattle 3Institute for Reproductive Biology University of Veterinary Medicine Hannover, B\u00fcnteweg 15, D-30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Helms","sequence":"additional","affiliation":[{"name":"Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants 2Clinic for Cattle 3Institute for Reproductive Biology University of Veterinary Medicine Hannover, B\u00fcnteweg 15, D-30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evrim","family":"Sahin","sequence":"additional","affiliation":[{"name":"Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants 2Clinic for Cattle 3Institute for Reproductive Biology University of Veterinary Medicine Hannover, B\u00fcnteweg 15, D-30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinrich","family":"Bollwein","sequence":"additional","affiliation":[{"name":"Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants 2Clinic for Cattle 3Institute for Reproductive Biology University of Veterinary Medicine Hannover, B\u00fcnteweg 15, D-30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edda","family":"T\u00f6pfer-Petersen","sequence":"additional","affiliation":[{"name":"Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants 2Clinic for Cattle 3Institute for Reproductive Biology University of Veterinary Medicine Hannover, B\u00fcnteweg 15, D-30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagmar","family":"Waberski","sequence":"additional","affiliation":[{"name":"Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants 2Clinic for Cattle 3Institute for Reproductive Biology University of Veterinary Medicine Hannover, B\u00fcnteweg 15, D-30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,4,1]]},"reference":[{"key":"2026010713133999500_bib1"},{"key":"2026010713133999500_bib2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1046\/j.1439-0531.2003.00419.x","article-title":"Assessing in vivo fertilizing capacity of liquid-preserved boar semen according to the Hanover Gilt Model","volume":"38","author":"Ard\u00f3n","year":"2003","journal-title":"Reproduction in Domestic Animals"},{"key":"2026010713133999500_bib3","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1016\/j.theriogenology.2004.08.008","article-title":"Accessory sperm: a biomonitor of boar sperm fertilization capacity","volume":"63","author":"Ard\u00f3n","year":"2005","journal-title":"Theriogenology"},{"key":"2026010713133999500_bib4","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1095\/biolreprod36.4.915","article-title":"Heterogeneity of sperm nuclear chromatin structure and its relationship to bull fertility","volume":"36","author":"Ballachey","year":"1987","journal-title":"Biology of Reproduction"},{"key":"2026010713133999500_bib5","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1095\/biolreprod59.6.1275","article-title":"Mammalian fertilization misread? 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