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Cast age ewes were fed a ration providing 0.5\u00d7 (0.5\u200aM) or 1.5\u00d7 (1.5\u200aM) live weight maintenance requirements for 2 weeks before slaughter. The ewes were synchronized and superovulated with FSH and pregnant mares serum gonadotropin. At slaughter, oocytes and granulosa cells were aspirated from follicles &amp;gt;2\u200amm in diameter and the relative abundance of 8 and 17 transcripts in oocytes and granulosa cells respectively were analyzed by semi-quantitative RT-PCR. In the oocytes, no differences between groups were observed for five transcripts (GDF9, BMP15, c-kit, glucose transporter 1 (SLC2A1), and hexokinase 1), but a lower amount of glucose transporter 3 (SLC2A3), sodium\/glucose cotransporter 1 (SLC5A1), and Na+\/K+ ATPase mRNAs was detected in the 0.5\u200aM group. Increased expression of PTGS2, HAS2, and the leptin receptor long form was observed in granulosa cells from the 0.5\u200aM group. No differences between groups were observed for the other transcripts (early growth response factor-1, estrogen receptor-\u03b1, LH and FSH receptors, gremlin 1, pentraxin 3, KIT ligand, glucose transporters 1, 3, and 8, IGF1, IGF1 receptor, leptin receptor, and tumor necrosis factor-stimulated gene 6). Expression of leptin and sodium\/glucose cotransporter 1 was not detected in both groups. The present data indicate that pre-mating nutrition is associated with alteration in the mRNA content in oocytes and surrounding follicle cells in ewes, which may account for the reduced reproductive performance typical of ewes that are fed a restricted ration for a short period of time before mating.<\/jats:p>","DOI":"10.1530\/rep-07-0394","type":"journal-article","created":{"date-parts":[[2008,5,30]],"date-time":"2008-05-30T20:44:55Z","timestamp":1212180295000},"page":"303-312","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":60,"title":["Effects of pre-mating nutrition on mRNA levels of developmentally relevant genes in sheep oocytes and granulosa cells"],"prefix":"10.1093","volume":"136","author":[{"given":"Laura F","family":"Pisani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Antonini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Pocar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana A L","family":"Brevini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart M","family":"Rhind","sequence":"additional","affiliation":[{"name":"Laboratory of Biomedical Embryology Department of Animal Science, University of Milan, Via Celoria 10, 20133 Milano, Italy 1The Macaulay Institute Craigiebuckler, Aberdeen, AB15 8QH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulvio","family":"Gandolfi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,9,1]]},"reference":[{"key":"2026010712471013600_bib1","first-page":"403","article-title":"Interactions between nutrition and ovarian activity in cattle: physiological, cellular and molecular mechanisms","volume":"61","author":"Armstrong","year":"2003","journal-title":"Reproduction Supplement"},{"key":"2026010712471013600_bib2","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/mrd.1099","article-title":"Glucose transporter expression is developmentally regulated in in vitro derived bovine preimplantation embryos","volume":"60","author":"Augustin","year":"2001","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712471013600_bib3","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.domaniend.2005.02.024","article-title":"Leptin: a metabolic signal affecting central regulation of reproduction in the pig","volume":"29","author":"Barb","year":"2005","journal-title":"Domestic Animal Endocrinology"},{"key":"2026010712471013600_bib4","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.theriogenology.2007.04.045","article-title":"The effect of in vitro treatment of bovine embryos with IGF1 on subsequent development in utero to day 14 of gestation","volume":"68","author":"Block","year":"2007","journal-title":"Theriogenology"},{"key":"2026010712471013600_bib5","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/S0093-691X(01)00485-X","article-title":"Effect of nutrition on endocrine parameters, ovarian physiology, and oocyte and embryo development","volume":"55","author":"Boland","year":"2001","journal-title":"Theriogenology"},{"key":"2026010712471013600_bib6","first-page":"1593","article-title":"Effects of plane of nutrition on in vitro fertilization and early embryonic development in sheep","volume":"84","author":"Borowczyk","year":"2006","journal-title":"Theriogenology"},{"key":"2026010712471013600_bib8","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1002\/mrd.20140","article-title":"Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos","volume":"69","author":"Brevini","year":"2004","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712471013600_bib7","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/S0093-691X(01)00481-2","article-title":"The maternal legacy to the embryo: cytoplasmic components and their effects on early development","volume":"55","author":"Brevini Gandolfi","year":"2001","journal-title":"Theriogenology"},{"key":"2026010712471013600_bib9","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1210\/endo.137.2.8593808","article-title":"Metabolic interfaces between growth and reproduction. 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