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Relative entry rates of cryoprotective agents (CPAs) were ethylene glycol\u2248DMSO&amp;gt;propane-1,2-diol&amp;gt;glycerol&amp;gt;propane-1,3-diol. More polar compounds including major epididymal secretions were impermeant. None of the compounds entered spermatozoa through quinine-sensitive channels; rather, quinine increased the size of solute-swollen spermatozoa, suggesting that regulatory volume decrease and osmolyte loss occurred under these conditions. Volume responses to lowered osmolality revealed a greater volume-regulating ability of spermatozoa from the B6D2F1 strain than the C57BL6 strain. As the former strain displays better post-thaw fertility, their spermatozoa may have greater osmolyte loads enabling them to cope better with osmotic stress. Inadequate volume regulation, due to CPA-induced osmolyte loss, may affect post-thaw fertility. Knowing the permeability towards cryoprotectants will help to make a better choice of CPAs that are less damaging to sperm during cryopreservation.<\/jats:p>","DOI":"10.1530\/rep-07-0573","type":"journal-article","created":{"date-parts":[[2008,4,11]],"date-time":"2008-04-11T11:48:19Z","timestamp":1207914499000},"page":"625-633","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":16,"title":["The tonicity of murine epididymal spermatozoa and their permeability towards common cryoprotectants and epididymal osmolytes"],"prefix":"10.1093","volume":"135","author":[{"given":"T G","family":"Cooper","sequence":"first","affiliation":[{"name":"Institute of Reproductive Medicine of the University Domagkstrasse 11, D-48129 M\u00fcnster, Germany 2Department of Biological Sciences University of New Orleans, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J 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